rna Pol II is the focus of eukaryotic transcription, because it is the most studied polymerase, and is also responsible for transcribing most genes-indeed, essentially all protein-encoding genes a RNA Pol I transcribe the large ribosomal RNa precursor gene a RNA Pol II transcribe tRNA gene, some small nuclear RNA genes and the 5s rRNA genes 11
11 ◼ RNA Pol II is the focus of eukaryotic transcription, because it is the most studied polymerase, and is also responsible for transcribing most genes-indeed, essentially all protein-encoding genes ◼ RNA Pol I transcribe the large ribosomal RNA precursor gene ◼ RNA Pol II transcribe tRNA gene, some small nuclear RNA genes and the 5S rRNA genes
Table 12-1: the subunits of RNA polymerases Prokaryotic Eukaryotic Bacterial Archaeal RNAP I RNAP lI RNAPⅢ Core Core (Pol D) Pol Ill) A/A RPA1 RPB1 RPC1 RPA2 RPB2 RPC2 BDLK RPC5 RPB3 RPC5 RPC9 RPB11 RPC9 RPB6 RPB6 RPB6 [+6 others [+9 others [+7 others [+11 others 12
12 Table 12-1: The subunits of RNA polymerases
The bacterial RNa polymerase The core enzyme alone synthesizes RNA 13
13 The bacterial RNA polymerase The core enzyme alone synthesizes RNA a a b b’ w
prokaryotic Fig 12-2 RNAP Comparison The same color indicate the homologous of the two enzymes eukaryotic a3CRPB2 RPB3 RPB1 RPB11 RPB6 14
14 a a b’ w RPB3 RPB11 RPB2 RPB1 RPB6 Fig 12-2 RNAP Comparison The same color indicate the homologous of the two enzymes prokaryotic eukaryotic b
Crab claw"shape of RNAP (The shape of DNA pol is Active center cleft 15
15 “Crab claw” shape of RNAP (The shape of DNA pol is__) Active center cleft