B
Uracil (from substrate) Threonine side chain serine e side chain FIGURE 8.8 Hydrogen bonds between an enzyme and substrate. The enzyme ribonuclease forms hydrogen bonds vith the uridine component of the substrate
nzyme-substrate complex Tyr 96 PeAYOYeY Phe 87 va|247 Asp 297 Leu 244 Camphor(substrate) va|295 Heme FIGURE 8.5 Structure of an enzyme-substrate complex(Left) The enzyme cytochrome P-450 is illustrated bound to its substrate camphor. (Right) In the active site the substrate is surrounded by residues from the enzyme. Note also the presence of a heme cofactor
Enzyme-substrate complex
Two models -enzyme bind its substrate Lock and key model锁-钥: Emil Fischer,1894 · Induced fit model诱导契合: Danie|E Koshland jr 1958
Two models---enzyme bind its substrate • Lock and key model锁-钥:Emil Fischer,1894 • Induced fit model诱导契合:Daniel E. Koshland,Jr 1958
Lock and key model Substrate b Active site ES complex Enzyme
Lock and key model