些常用的型限制性内切酶 Recognition Sequences for Some Type Il Restriction Endonucleases Hind IIl (5)AAGCTT(3) BamHI (5)GG ATCC(3) TTCGAA CCTAGG (5)GCGGCCGC(3' (5)ATCGAT(3) CGCCGGCG TAGCTA (5)CTGCAG(3) ECORI (5)GAA TC(3) GACGTC CTTAAG Pvull (5')CAGCTG(3) ECORV (5”) GATATO(3") GTCGAC CTATAG ThiLl (5) GACNNNG TO(3”) Haelll (5)GGcc(3) CTGNNNCAG C CGG 个 Arrows indicate the phosphodiester bonds cleaved by each from which it is derived (e g-, Bam for Bacillus am loli- estriction endonuclease. Asterisks indicate bases that are quefaciens, Eco for Escherichia coli). the Roman numerals methylated by the corresponding methylase(where known). included in the enzyme names (e. g, BamHi) distinguish N denotes any base. Note that the name of each enzyme different restriction endonucleases isolated from the same consists of a three-letter abbreviation of the bacterial species bacterial species rather than the type of restriction enzyme
一些常用的II型限制性内切酶
Dimeric structure of type II restriction endonucleases
Dimeric structure of type II restriction endonucleases
Cleavage Recognition Cleavage site sequences site Chromosomal DNa GGTGAATTCAGCTTCGCATTAGCAGCTGTAGC -CCACTTAAGTCGAAGCGTAATCGTCIGACATCG EcoRI PuuII restriction restrction endonuclease endonuclease GGTG AATTCAGCTTCGGATTAGCAG CTGTAGC - CCACTTAA GTCGAAGCGTAATCGTC GACATCG Sticky endis Blunt ends 粘性未端,也叫 (b) cohesive end 平滑未端 DNA li Plasmid cloning vector cleaved with EcoRl and poull
粘性末端，也叫 cohesive end 平滑末端
Psti HindIlI BamHI Smal AATTCCTGCAGAAGCTTCCGGATCCCCGG G GGACGTCTTCGAAGGCCTAGGGGCCCTTAA ynthetic polylinker DNA lis gase Plasmid cloning vector cleaved with EcorI BamHI S polylinker 入
在 克 隆 载 体 中 导 入 更 多 的 酶 切 位 点
画DNA聚合 最常用的DNA聚合酶有以下4种 DNA聚合酶I DNA聚合酶I大片段 Klenow片段 Taq dna聚合酶 T4噬菌体DNA聚合酶
最常用的DNA聚合酶有以下4种 DNA聚合酶Ⅰ DNA聚合酶Ⅰ大片段——Klenow片段 Taq DNA聚合酶 T4 噬菌体DNA聚合酶 DNA聚合酶