(a)Primer extension reaction: TACTATGCCAGA- 21-base primer Replication with ddTTP TACTATGCCAGA (26 bases) ATGAD
(b)Products of the four reactions: Products of ddA rxn Products of ddC rxn Template:. TACTATGCCAGA_ Template: TACTATGCCAGA_ (22) 0 (28) ATGATA (25) ATGA 32) (27） ATGATACGGTO ATGATA
Products of ddG rxn Products of ddT rxn Template: TACTATGCCAGA_ Template: TACTATGCCAGA (24) ATO (23) AD 29) ATGATAC (26) ATGAD (30) ATGATACG 31) ATGATACGGO 33) ATGATACGGTCO
(c)Electrophoresis of the products: ddA ddC ddG ddT 一 T OH 二 G G 一 A G A
ACGT Figure 5.19 A typical sequencing film.The sequence begins CAAAAAACGG.You can probably read the rest of the sequence to the top of the film.(Source:Courtesy Life Technologies,Inc.,Gaithersburg.MD.)