Table 19.2 Proofreading reduces the errors made by DNA polymerases. 日nzyme Synthetic Proofreading 日ror rate domain domain proof +proof Ecoli DNApol I aa 200-600 N-terminal 105 5×107 Ecoli DNA pol a subunit a subunit 7×106 5X109 T4 DNA C-terminal pol N-terminal 5×105 107 T7 DNA pol 118-145 105 106 Reverse transcript None 105 ase
Tble 193 There are 5 nammnlian INApolynerases (ientifed by Geekletters;yeastequsvalents are given Rnan mneraks). A a() 6(红II) 8(II) ￥ Polnerase Locaton Ncear Ncear Ncear Ncear Mtochondti al Furction Lacgig Leai血rg Repair Repair Repication strand stand synthesissynthesis pi童g Rehtive 80% 10-15% 2-15% activity Mss(dalo 300,000 170 250,000 40,000 180 Is) 230,000 300.000 Sbunits Catalytic Catalytic Catalytic Catalytic Catalytic core core core core 2 prinnse 1 unlnown 1 urknown 2 unknown 1 uknown 1eg重es PONA 3'5 b Ys s b s e知nceas e Dideoxythy No effect N effect Wak hhbiory ihbiory nilire pi並on hhbiory Ihbiory nhbiory N effect No effect
Flgure 19.4 3
↓ Synthesis of RNA prime L Extension of Okatoki frogment ↓ m ↓ Removal of RNA primer HHHH出my ↓ Gop is filled,ond nick is soaled