537Alul3*55*37Haelll3553BamHI35*537Hindill5*3*3>5EcoRI35.AlulandHaelllproducebluntendsBamHI HindlllEcoRIproduce"sticky"endsandXSchool of Life Sciences Tsinghua University
Type III restriction endonuclease:Recognize specific nucleic acid sequencebut the specific site is not palindrome. Incisedouble strain DNA in specific site with severalnucleoside pairs away from the recognizednucleic acid sequence. These severalnucleoside pairs do not have specificity. Sotype III restriction endonuclease is not used ingene cloningXSchool of Life Sciences Tsinghua University
Type III restriction endonuclease: • Recognize specific nucleic acid sequence, but the specific site is not palindrome. Incise double strain DNA in specific site with several nucleoside pairs away from the recognized nucleic acid sequence. These several nucleoside pairs do not have specificity. So type III restriction endonuclease is not used in gene cloning