BIOSAFETY LEVEL HEPA= high-efficiency particulate air 2 Isolation of laboratory No No Ye Yes Room sealable for decontamination No No Yes Yes Ventilation inward airflow No Desirable ye es controlled ventilating system No Desirable Yes Yes HEPA-filtered air exhaust No No Yes/No Yes Double-door ent No No Ye Yes Airlock No No No es Airlock with shower No No No Yes Anteroom No No Yes Anteroom with shower No No Yes /No°No Effluent treatment No No Yes/No°Yes Autoclave on site No Desirable Yes Yes in laboratory room No No Desirable Yes double-ended NoNo Desirable Yes Biological safety cabinets No Desirable Yes Yes Personnel safety monitoring capability No No Desirable Yes
aa environmental and functional isolation from general traffic. ab Dependent on location of exhaust. ue Dependent on agent(s)used in the laboratory od For example, window, closed-circuit television, two-way communication THus, the assignment of a biosafety level takes into consideration the organism(pathogenic agent) used, the facilities available, and the equipment practices and procedures required to conduct work safely in the laboratory
❑a Environmental and functional isolation from general traffic. ❑b Dependent on location of exhaust. ❑c Dependent on agent(s) used in the laboratory. ❑d For example, window, closed-circuit television, two-way communication. ❑Thus, the assignment of a biosafety level takes into consideration the organism (pathogenic agent) used, the facilities available, and the equipment practices and procedures required to conduct work safely in the laboratory
Chapter 2. Microbiological risk assessment 口2.1 Features UThe backbone of the practice of biosafety is risk assessment aWhile there are many tools available to assist in the assessment of risk for a given procedure or experiment, the most important component is professional judgement available
Chapter 2. Microbiological risk assessment ❑2.1 Features ❑The backbone of the practice of biosafety is risk assessment. ❑While there are many tools available to assist in the assessment of risk for a given procedure or experiment, the most important component is professional judgement available
Risk assessments should be performed by the individuals most familiar with: u the specific characteristics of the organisms being considered for use, u the equipment and procedures to be employed, u animal models that may be used, and u the containment equipment and facilities
Risk assessments should be performed by the individuals most familiar with: ❑ the specific characteristics of the organisms being considered for use, ❑ the equipment and procedures to be employed, ❑ animal models that may be used, and ❑ the containment equipment and facilities
The la boratory director or principal investigator is responsible for ensuring that adequate and timely risk assessments are performed, and for working closely with the institutions safety committee and biosafety personnel to ensure that appropriate equipment and facilities are available to support the work being considered
The laboratory director or principal investigator is responsible for ensuring that adequate and timely risk assessments are performed, and for working closely with the institution’s safety committee and biosafety personnel to ensure that appropriate equipment and facilities are available to support the work being considered