Note #1 Detailed balance k +1 k k tn P、P1 p 1 dip i k,[P][S]+k[P1] dt +1 d[P1] k [P1][S]+k2[P]+k,1[P][S] +211 1 k1[P1] dt k,[P,][S]+k[ +2 etc k [P.] K≡-+j [P [S] 1
Note #1 Detailed balance P o P 1 P 2 ... Pn-1 P n k+1 k-1 k+2 k-2 k+n k-n ] 2 [P 2 ][S] k 1 [P 2 k ] 1 [P 1 ][S] k o [P 1 ] k 2 [P 2 ][S] k 1 [P 2 k dt ] 1 d[P 0 ] 1 [P 1 ][S] k o [P 1 k dt ] o d[P 0 − + + = − = − − + + − + + = = − − + + = = − ][S] j 1 [P ] j [P j k j k j K − = − + ≡ etc
I Identical and independent binding sites k k k k K=k/k K1=2K K2=K/2 2K[S]+2K4[S 2K[S] use Adair: r 1+2K[S]+K4[S 1+K[S]
I Identical and independent binding sites k + S k + k - k - S S k + k + k - S k - K=k +/k - K 1=2K K 2=K/2 1 K[S] 2K[S] 2 [S] 2 1 2K[S] K 2 [S] 2 2K[S] 2K r + = + + + use Adair: =
II Non-identical and independent binding sites k k k K=k/k K=kA/k KISI K IS] Independent binding: r 1+K[S]1+K[S]
II Non-identical and independent binding sites S S S k + k - k + * k - * Independent binding: [S] * 1 K [S] * K 1 K[S] K[S] r + + + = K=k +/k - K *=k + */k - * S k + * k - * k + k -
iii Identical and interacting binding sites k k k K=k/k K1=2K K2=K*/2 K*=k*/k_ 2K [S]+2KK [S use Adair r 1+2K[S]+KK[S]
III Identical and interacting binding sites S k + * k - * k + k - K=k +/k - K 1=2K K 2=K */2 K *=k + */k - * S S S k + k - k + * k - * 2 [S] * 1 2K[S] KK 2 [S] * 2K[S] 2KK r + + + use Adair: =