《医学遗传学》课程PPT教学课件（留学生 Medical Genetics）11 Chapter Therapy of the Hereditary Disease

Gene Delivery Method *Ex vivo Controlled process;transfected cells are selected and expanded;more manipulations Cells are usually autologous;they are then returned back to the patient

 Controlled process; transfected cells are selected and expanded; more manipulations  Cells are usually autologous; they are then returned back to the patient Gene Delivery Method ❖ Ex vivo

Physical Methods Direct injections Electroporation Microparticle bombardment (gene gun

Physical Methods ❖ Direct injections ❖ Electroporation ❖ Microparticle bombardment (gene gun)

Chemical Methods 必 Coprecipitation with calcium phosphate Transfer efficiency:1%~0.1%

Chemical Methods ❖ Coprecipitation with calcium phosphate  Transfer efficiency: 1% ~0.1%

Membrane Fusion Artificial liposomes Erythrocyte ghosts Microcell Spheroplast

Membrane Fusion ❖ Artificial liposomes ❖ Erythrocyte ghosts ❖ Microcell ❖ Spheroplast