The Winogradsky column Lake or Algae and cyanobacteria Purple nonsulfur bacteria Mud suDo emented Patches of purple sulfur or ath organIc green sulfur bacteria ntrients and Anoxic decomposition and sulfate reduction H2s The column is filled with organic-rich, preferably sulfide-containing, mud. Hay. shredded newsprint, sawdust, shredded leaves or roots, ground meats, hard boilded eggs, and even dead animals are added. CaCO3 and CaSo4 as buffer are added too
The Winogradsky column The column is filled with organic-rich, preferably sulfide-containing, mud. Hay, shredded newsprint, sawdust, shredded leaves or roots, ground meats, hard boilded eggs, and even dead animals are added. CaCO3 and CaSO4 as buffer are added too
Agar shake tube method: Isolation of anaerobic bactetia in pure culture
Agar shake tube method: Isolation of anaerobic bactetia in pure culture
Identification and Quantification: Nucleic Acid Probes, Fluorescent Antibodies, and viable counts Microautoradiography of single cells of Bacillus megaterium hybridized with 16S rRNA of bacteria (left) and to the 18S rRNA of Eukatya(right)
Identification and Quantification: Nucleic Acid Probes, Fluorescent Antibodies, and Viable Counts Microautoradiographs of single cells of Bacillus megaterium hybridized with 16S rRNA of bacteria (left) and to the 18S rRNA of Eukatya (right)
Identification and Quantification: Nucleic Acid Probes. Fluorescent Antibodies and viable counts fluorescently labeled rRNA probes Left, phase contrast photomicrograph of B Megaterium and the yeast Saccharomyces cerevisiae (no probe). Center, same field, cells stained with universal IRNA probe. Right, same field, cells stained with eukaryal probe
Identification and Quantification: Nucleic Acid Probes, Fluorescent Antibodies, and Viable Counts Fluorescently labeled rRNA probes. Left, phase contrast photomicrograph of B. Megaterium and the yeast Saccharomyces cerevisiae (no probe). Center, same field, cells stained with universal rRNA probe. Right, same field, cells stained with eukaryal probe
Identification and Quantification: Nucleic Acid Probes. Fluorescent Antibodies and viable counts Differentiation of closely related gram-negative bacteria Left, phase micrograph of mixture of Proteus vulgaris and a related bacterium isolated from wasps; Center, same field stained with the bacterial probe Right, same field stained with a probe specific for the bacterium from wasps
Identification and Quantification: Nucleic Acid Probes, Fluorescent Antibodies, and Viable Counts Differentiation of closely related gram-negative bacteria. Left, phase micrograph of mixture of Proteus vulgaris and a related bacterium isolated from wasps; Center, same field stained with the bacterial probe; Right, same field stained with a probe specific for the bacterium from wasps