KidsofTestingofSARSvirusTherecombinationalproteinofSARSvirusHuman serum(containingtheantibodyofSARSvirus?)(differentdilutionofserum)Polyclonal antibody againstHumanlgG-HRP(horseradishperoxidase)Substratesolution,OD490nm
Kids of Testing of SARS virus ⚫ The recombinational protein of SARS virus ⚫ Human serum(containing the antibody of SARS virus?) (different dilution of serum) ⚫ Polyclonal antibody against HumanIgG-HRP (horseradish peroxidase) ⚫ Substrate solution, OD490nm
Kidsof TestingofHBsAgMonoclonalantibodyagainstHBsAg(anti-HBsAgantibody)Humanserum(containing HBsAg?)Polyclonal antibodyagainstHBsAg-HRP(horseradishperoxidase)(anti-HBsAg-HRP)Substratesolution,OD450nm
Kids of Testing of HBsAg ⚫ Monoclonal antibody against HBsAg(antiHBsAg antibody) ⚫ Human serum(containing HBsAg?) ⚫ Polyclonal antibody against HBsAgHRP(horseradish peroxidase)(antiHBsAg-HRP) ⚫ Substrate solution, OD450nm
OurTestofELISAAntigen:HumanIgG(5μg/well)·Firstantibody:Rabbitanti-humanIgGantiserum(serumdilution)Secondaryantibody:Goatanti-rabbitIgGHRP(1:20,000)
Our Test of ELISA ⚫ Antigen: Human IgG (5mg/well). ⚫ First antibody: Rabbit anti-human IgG antiserum (serum dilution). ⚫ Secondary antibody:Goat anti-rabbit IgGHRP (1:20,000)