鉴别实验 有机氟一呈色反应(经氧瓶燃烧后生成无机氟化物,再与茜素氟蓝及 硝酸亚铈呈色) 有机氯—沉淀反应(经有机破坏生成无机氯化物,在硝酸酸性下与硝 酸银作用,生成白色沉淀) 酚羟基一呈色反应(与重氮苯磺酸反应生成红色偶氮染料) 制备衍生物测定其熔点 炔基一沉淀反应(与硝酸银反应生成白色沉淀) 酯基一水解产物的反应 Section 2
鉴 别 实 验 Section 2 有机氟 — 呈色反应(经氧瓶燃烧后生成无机氟化物，再与茜素氟蓝及 硝酸亚铈呈色） 有机氯 — 沉淀反应(经有机破坏生成无机氯化物，在硝酸酸性下与硝 酸银作用，生成白色沉淀) 酚羟基 — 呈色反应 (与重氮苯磺酸反应生成红色偶氮染料) — 制备衍生物测定其熔点 酯基 — 水解产物的反应 炔基 — 沉淀反应 (与硝酸银反应生成白色沉淀)
<Previous Identification tests Organic fluorine Color reaction( with alizarin complexone and Cerous nitrate after destroyed by oxygen flask combustion Organic chlorine - Precipitation reaction( with nitric acid and ilver nitrate after destroyed by oxygen flask combustion) Phenolic hydroxyl-Color reaction(diazobenzenesul fonic acid) Melting point determination of derivatives Alkynyl group- Precipitation reaction( with silver nitrate) Ester group y irolysis product reaction Section 2
Identification tests Section 2 Organic fluorine — Color reaction(with alizarin complexone and Cerous nitrate after destroyed by oxygen flask combustion ） Organic chlorine — Precipitation reaction(with nitric acid and silver nitrate after destroyed by oxygen flask combustion) Phenolic hydroxyl — Color reaction (diazobenzenesulfonic acid) — Melting point determination of derivatives Ester group — Hydrolysis product reaction Alkynyl group — Precipitation reaction(with silver nitrate)
Reaction with sulfuric acid Adding wa- Names olor Fluorescence ter dilution Cortisone Yellow or Color disappears and acetate clear solution formed orange Yellow to Prednisone Orange blue-green
Names Color Fluorescence Adding water dilution Cortisone acetate Yellow or orange - Color disappears and clear solution formed Prednisone Orange - Yellow to blue-green Reaction with sulfuric acid
Ultraviolet spectrophotometr i Conjugated bonding systems 1.aB-unsaturated ketone group 2. Steroids with aromatic ring A
Ultraviolet spectrophotometry Conjugated bonding systems: 1.α,β-unsaturated ketone group 2. Steroids with aromatic ring A
OH 2.0 HO OH 1.5 sorbanc 1.0 Hydrocortisone Betamethasone Hydrocortisone OH e05人 HO OH 200 250 300 350 Betamethasone The U\ spectra of hydrocotisone and betamethasone d
The UV spectra of hydrocotisone and betamethasone