Epidemiology 4th leading cause of death from parasitic diseases worldwide organism t of deaths/yr infected Entamoeba ~75,000 300 million Ascaris ~200.000 480 million Schistosoma ~750.000 c200 million Plasmodium 2-3 million -500 million (Malaria) Amoebiasis is not restricted to the tropics and subtropics, it also occurs in temperate and even in arctic and antarctic zones
Epidemiology • 4th leading cause of death from parasitic diseases worldwide • Organism # of deaths/yr # infected • Entamoeba ~75,000 ~300 million • Ascaris ~200,000 ~480 million • Schistosoma ~750,000 ~200 million • Plasmodium 2-3 million ~500 million • (Malaria) • Amoebiasis is not restricted to the tropics and subtropics, it also occurs in temperate and even in arctic and antarctic zones
Trophozoite Cyst granular nested RBC endoplasm chromatoid body karyosome 际 :t4 ectoplasm gM nucleus karyosome nucleus 10黑 Entamoeba histolytica
granular endoplasm ectoplasm Entamoeba histolytica
Morphology Trophozoite Sze:10-40m Shape: ovoid with pseudopodium >Basic structure: cytoplasm, vesicular nucleus ( chromatin granules, nuclear membrane, karyosome)
Morphology ▪ Trophozoite ➢Size: 10-40 m ➢Shape: ovoid with pseudopodium ➢Basic structure: cytoplasm, vesicular nucleus (chromatin granules, nuclear membrane, karyosome)
E histolytica trophozoite Ingested endoplasm RBC Nucleus with central karyosome and fine regular granules( peripheral chromatin granules), evenly distributed around the nuclear membrane Ectoplasm, P seudopod E histolytica trophozoite
Ingested RBC Nucleus with central karyosome and fine regular granules(peripheral chromatin granules), evenly distributed around the nuclear membrane Ectoplasm, Pseudopod E. histolytica trophozoite endoplasm E. histolytica trophozoite
E histolytica Stages TROPHOZOITES Cause amoebiasis(damage tissue) Spread throughout the body Rarely transmit the infection to others Labile in liquid stools or tissue, and must be rapidly found or preserved quick fixation cold storage) for DX
E. histolytica Stages - TROPHOZOITES ▪ Cause amoebiasis (damage tissue) ▪ Spread throughout the body ➢Rarely transmit the infection to others ▪ Labile in liquid stools or tissue, and ➢must be rapidly found or preserved (quick fixation & cold storage) for Dx