His HG 61 HisHca His Hc3 r state R state
Val Fg5 Heme Le FG3 His F8 Helix f Leu F4 T state R state
Oo in pO, in tissues 1.0 lungs High-affinity state 0.8 Transition from low- to high affinity state 0.6 0.4 Low-affinity 0.2 state 12 16 pO2(kPa) kPa: kilopascal, I atm=760 mm Hg= 14.7 psi=101.3 kPa
kPa: kilopascal, 1 atm = 760 mm Hg = 14.7 psi = 101.3 kPa
1.0 00.5 K 10 [L](arbitrary units) (a)
binding sites occupied L total binding sites LL+Kd e is a measurable quantity in experiment. LL is the free ligand concentration
binding sites occupied [L] = ------------------------------- = ------------- total binding sites [L] + Kd is a measurable quantity in experiment. [L] is the free ligand concentration