R96 Q183 OH. N Q94 N 168RT203 R N R Gly67 N Q69 H148>N OH2 H2O R 146 R R s205 E222 7 Schematic diagram of the interactions between the chromophore and its surroundings in the S65T mutant. Possible hydrogen bonds are drawn as dashed lines K Brejc et al PNAS USA 1997, 94, 2306
K.Brejc et.al PNAS USA 1997, 94, 2306
p-HBDI showed weak fluorescence HO N△N-R N\N-CH3 N△N-CH N△N-CH3 CH3 1 R= CO2Et CH3 CH 2R= CH 3 5 Ethanol solutions of 2, 3, 4, and 5 showed only very weak fluorescence at room temperature, Satoshi Kojima, et al. Tetrahedron Letters 1998, 39, 5239
Satoshi Kojima, et.al. Tetrahedron Letters 1998, 39, 5239 p-HBDI showed weak fluorescence
Temperature dependence of the fluorescence spectrum of p-HBDI 8x106 77K 7×106 90K 500≥58c0 ··100K 6x10 110K 5x1 120K 3X10 三1×10 400420440460480500520540560580 Wavelength /nt Fig. 2. Temperature dependence of the fluorescence spectrum of HBDI in ethanol Haruki niwa et al Proc. Natl. Acad. Sci. USA 1996.93. 13617
Haruki niwa et,al Proc. Natl. Acad. Sci. USA 1996 ,93, 13617 Temperature dependence of the fluorescence spectrum of p-HBDI
An important question How does the protein convert such a poor fluorophore o the brightly fluorescent GFP? a clear answer to this question could assist in designing brighter GFP variants
An important question ◼ How does the protein convert such a poor fluorophore to the brightly fluorescent GFP? ◼ A clear answer to this question could assist in designing brighter GFP variants
2, Experiment research
2, Experiment research