CHATER 17 Gene Regulation in eukaryotes 、真核的转录激活蛋白的结构特征 The structure features of the eukaryotic transcription activators Topic 1: Conserved Mechanisms of Transcriptional Regulation from yeast(酵母)to Mammals(哺乳动物) 劣溪字 Wuhan University
12 Topic 1: Conserved Mechanisms of Transcriptional Regulation from Yeast (酵母) to Mammals (哺乳动物) CHAPTER 17 Gene Regulation in eukaryotes 一、真核的转录激活蛋白的结构特征 The structure features of the eukaryotic transcription activators
The basic features of gene requlation are the same in all eukaryotes, because of the similarity in their transcription and nucleosome structure e yeast is the most amenable to both genetic and biochemical dissection, and produces much of knowledge of the action of the eukaryotic repressor and activator o The typical eukaryotic activators works in a manner similar to the simplest bacterial Case e Repressors work in a variety of ways
13 The basic features of gene regulation are the same in all eukaryotes, because of the similarity in their transcription and nucleosome structure. Yeast is the most amenable to both genetic and biochemical dissection, and produces much of knowledge of the action of the eukaryotic repressor and activator. The typical eukaryotic activators works in a manner similar to the simplest bacterial case. Repressors work in a variety of ways
1. Eukaryotic activators(真核激活蛋白)have separate dna binding and activating functions 【与原核相似】, which are very often on separate domains of the protein. activation domain DNA-binding DNA domain DNA-binding site Fig. 17-2 Gal4 bound to its site on DNA 4
14 1. Eukaryotic activators (真核激活蛋白) have separate DNA binding and activating functions 【与原核相似】, which are very often on separate domains of the protein. Fig. 17-2 Gal4 bound to its site on DNA
Eukaryotic activators---Example 1: Gal4 y Gal4 is the most studied eukaryotic activator Y Gal4 activates transcription of the galactose genes in the yeast S. cerevisae Gal4 binds to four sites (UASc) upstream of GAL1 and activates transcription 1, 000-fold in the presence of galactose 23 4 GAL 1 UAS 275bp Fig. 17-3 The regulatory sequences of the yeast GALl gene
Fig. 17-3 The regulatory sequences of the Yeast GAL1 gene.15 Eukaryotic activators---Example 1: Gal4 ✓ Gal4 is the most studied eukaryotic activator ✓ Gal4 activates transcription of the galactose genes in the yeast S. cerevisae. ✓ Gal4 binds to four sites (UASG) upstream of GAL1, and activates transcription 1,000-fold in the presence of galactose
Eukaryotic activators---Example 1: Gal4 Experimental evidences showing that Gal4 contains separate DNA binding and activating domains 1. Expression of the N-terminal region(DNA binding domain) of the activator produces a protein bound to the DNA normally but did not activate transcription 2. Fusion of the C-terminal region(activation domain) of the activator to the dna binding domain of a bacterial repressor LexA activates the transcription of the reporter gene. Domain swap experiment 实验介绍系列1一 Experiment introduction series 16
16 Eukaryotic activators---Example 1: Gal4 Experimental evidences showing that Gal4 contains separate DNA binding and activating domains. 1. Expression of the N-terminal region (DNAbinding domain) of the activator produces a protein bound to the DNA normally but did not activate transcription. 2. Fusion of the C-terminal region (activation domain) of the activator to the DNA binding domain of a bacterial repressor, LexA activates the transcription of the reporter gene. Domain swap experiment 实验介绍系列1－Experiment introduction series