Soil fertilityincrease or decreaseAnalyses:soil management- Chemical-physicalsoil properties- GasChromatografy-Mass SpectrometrySoil organic matter-InfraredSpectroscopydegradation-LiquidChromatografyaccumulation(HPSEC)/stabilization-Liquid stateNMR- Solid state NMRHumic substancesIREEA-NanjingAgricultural University/April 2008
UNIVERSITY OF NAPOLI “FEDERICO II” AGRICULTURAL FACULTY Soil Chemistry Department Soil organic matter Dr. Spaccini Riccardo Soil fertility increase or decrease soil management Analyses: - Chemical-physical soil properties - GasChromatografy - Mass Spectrometry -InfraredSpectroscopy -LiquidChromatografy (HPSEC) - Liquid state NMR - Solid state NMR IREEA-Nanjing Agricultural University/ April 2008 Humic substances accumulation /stabilization degradation
IREEA-Nanjing Agricultural University/ April 2008OBJECTIVEMolecular characterization of soil humic acids extracted after soiltreatments with recycled organic biomassSpectroscopic analyses:IR-DRIFT (Diffuse Reflectance Infrared Fourier Transform)molecularcharacterizationoff-linepyrolysis withTetraMethylAmmoniumHydroxide(TMAH termpchemolysis)GasCromatografyMassSpectrometry
Molecular characterization of soil humic acids extracted after soil treatments with recycled organic biomass • IR-DRIFT (Diffuse Reflectance Infrared Fourier Transform) • Sequential extractions • CPMAS-13C-NMR (CrossPolarizationMagicAngleSpinningNuclear Magnetic Resonance Spectroscopic analyses: off-line pyrolysis with TetraMethylAmmoniumHydroxide (TMAH termochemolysis) OBJECTIVE GasCromatografy MassSpectrometry molecular characterization IREEA-Nanjing Agricultural University/ April 2008
Infrared (IR) Spectroscopymost spectroscopies technigues are based on the interaction betweenelectromagnetic wave and the unkown moleculeMicroNearFarUVVisInfraredwaveIRIR10-410-3≥ cm 10-510-210-125 μm5 μmenergythe energy associated with these wavelenght correspond to themolecular vibration of chemical bonds energyIREEA-NanjingAgriculturalUniversity/April2008
Infrared (IR) Spectroscopy most spectroscopies techniques are based on the interaction between electromagnetic wave and the unkown molecule UV Vis Far IR Infrared Near IR Micro wave λ cm 10−5 10−2 10−1 10−4 10−3 energy the most useful wavelenght range of electromagnetic radiation for infrared spectroscopy vary from 5 to 25 μm 25 μm 5 μm the energy associated with these wavelenght correspond to the molecular vibration of chemical bonds energy IREEA-Nanjing Agricultural University/ April 2008
Infrared Spectroscopyfrequency v = c/ 2 (Hz or s-1)wavelength 2 (cm)IR radiationinteraction withbond vibrationalHenergyIR signalsstretchingsimmetrycalasymmetricalHH福bendingHHin-planeout-of-planePIREEA-NanjingAgricultural University/April 2008
C Infrared Spectroscopy wavelength λ (cm) frequency ν = c/ λ (Hz or s-1) interaction with bond vibrational energy C H stretching H H C H H bending in-plane out-of-plane IR radiation C H simmetrycal asymmetrical C H IR signals IREEA-Nanjing Agricultural University/ April 2008
Asymmetrical stretchingSimmetrycal stretchingIn-plane bendingscissoringrockingOut-of-plane bendingtwistingwaggingIREEA-NanjingAgricultural University/April 2008
Simmetrycal stretching Asymmetrical stretching In-plane bending rocking scissoring Out-of-plane bending wagging twisting IREEA-Nanjing Agricultural University/ April 2008