UNIVERSITYOENAPOLL"FEDERICOII"AGRICULTURALFEACULTYSoil ChemistryDepartmentMolecular characterization of soil humic acidafter recycled organic biomass additionDr. Spaccini Riccardo
UNIVERSITY OF NAPOLI “FEDERICO II” AGRICULTURAL FACULTY Soil Chemistry Department Dr. Spaccini Riccardo Molecular characterization of soil humic acid after recycled organic biomass addition
OBJECTIVEMolecular characterization of soil humic acids extracted after soiltreatments with recycled organic biomassbulkcharacterizationSpectroscopicanalysesCPMAS13CNMR (CrossPolarizationMagicAngleSpinning)molecularcharacterizationSequential extractionsGasCromatografyMassSpectrometryIREEA-NanjingAgricultural University/ April 2008
Molecular characterization of soil humic acids extracted after soil treatments with recycled organic biomass • IR-DRIFT (Diffuse Reflectance Infrared Fourier Transform) GasCromatografy MassSpectrometry • Sequential extractions • CPMAS13CNMR (CrossPolarizationMagicAngleSpinning) Spectroscopic analyses: off-line pyrolysis with TetraMethylAmmoniumHydroxide (TMAH termochemolysis) bulk characterization molecular characterization OBJECTIVE IREEA-Nanjing Agricultural University/ April 2008
NMR spectroscopya spinning nucleus, with magnetic properties, ('Hi3C, 31P, 15N) possess a magnetic momentuuonce immersed in external strong field (B.) the magnetic momentof nuclei with a spin quantum number I = /2 will be lined up to B.withagainstorBo0Z0thereby assuming a circular path around the main z axis called precessionwith an angolar frequency (larmor frequency) tipical of each nucleusIREEA-NanjingAgricultural University/ April 2008
a spinning nucleus, with magnetic properties, (1H, 13C, 31P, 15N) possess a magnetic moment μ or thereby assuming a circular path around the main z axis called precession with an angolar frequency ω (larmor frequency) tipical of each nucleus with z ω against z ω once immersed in external strong field (B0) the magnetic moment of nuclei with a spin quantum number I = ½ will be lined up to B0 B0 NMR spectroscopy μ IREEA-Nanjing Agricultural University/ April 2008
4the various nuclei (e.g.'H) will have a randomdistribution along z axis;xfollowing the Boltzman distribution theynuclei aligned with BO have lower energyand are in slight excessMZoperating a vectorial sum, thetrasversal (X and Y)Xcomponents will cancel outyleaving a resultant vector Mrepresenting the total magneticmoment of those nucleiIREEA-NanjingAgricultural University/April 2008
y x z y x z the various nuclei (e.g.1H) will have a random distribution along z axis; operating a vectorial sum, the trasversal (X and Y) components will cancel out, leaving a resultant vector M representing the total magnetic moment of those nuclei following the Boltzman distribution the nuclei aligned with B0 have lower energy and are in slight excess M IREEA-Nanjing Agricultural University/ April 2008
Izthe application of a radiofrequency B1on the X axis with a frequency = to theXLarmor (o) frequency of the nuclei(resonance)ywill equalize the nuclei distribution alongB1Z+ and Z- directions.2.changing at the same time the phase ofXof sigular spinning nucleus that will beygrasped towards the Y- direction(phase coherence)B111IREEA-NanjingAgricultural University/April 2008
y x z the application of a radiofrequency B1 on the X axis with a frequency = to the Larmor (ω) frequency of the nuclei (resonance) will equalize the nuclei distribution along Z+ and Z- directions. .changing at the same time the phase of of sigular spinning nucleus that will be grasped towards the Y- direction (phase coherence) B1 y x z B1 IREEA-Nanjing Agricultural University/ April 2008