Rasi-RNAS Repeat associated small interfering rna (rasiRNA)is a class of small rna that is involved in the rna interference(RNAi) pathway. rasirNa is a subclass of Piwi-interacting RNAs (pIRNAs), which are smalI RNA molecule which interact with Piwi proteins. Piwi proteins are an argonaute subfamily rasirna associate with both the ago and Piwi argonaute protein subfamily while pirna only associates with the Piwi argonaute subfamily. In the germline, rasirna is involved in establishing and maintaining heterochromatin structure, controlling transcripts that emerge from repeat sequences and silencing transposons and retrotransposons
Rasi-RNAs Repeat associated small interfering RNA (rasiRNA) is a class of small RNA that is involved in the RNA interference (RNAi) pathway. RasiRNA is a subclass of Piwi-interacting RNAs (piRNAs), which are small RNA molecule which interact with Piwi proteins. Piwi proteins are an Argonaute subfamily. RasiRNA associate with both the Ago and Piwi Argonaute protein subfamily while piRNA only associates with the Piwi Argonaute subfamily. In the germline, RasiRNA is involved in establishing and maintaining heterochromatin structure, controlling transcripts that emerge from repeat sequences, and silencing transposons and retrotransposons
rasiRNA are Distinct fro other RNAi pathways Unlike miRNA and sirNa which function through the ago Argonaute protein subfamily, rasirna is aso distinction ts Piw size ontrary to miRNAs which are 21-23 nucleotides in lengt th siRNAs which are 20-25 nucleotides in length, and pirnas which are 24-31 nucleotides in length, rasiRNAs are 24-29 nucleotides in length depending on the organism it came from. Unlike siRNA which are derived from both the sense and antisense strand rasiRNa are derived from the antisense Interestingly, while miRNa requires Dicer-l for its production, and siRNa requires Dicer-2, rasirna does not require either; however, in some plants there are Dicer-like dcl) proteins that have been identified where Dell produces 24 nucleotide miRNA and sirNa while dcl2 produces 24 nucleotide rasiRNA. This research shows that not only is rasiRNa production distinct from miRNA and sirna, but that rasiRNA may be found in plants while piwi proteins are not
RasiRNA are Distinct from other RNAi pathways Unlike miRNA and siRNA which function through the Ago Argonaute protein subfamily, RasiRNA function through the Piwi Argonaute protein subfamily. RasiRNA is also distinct in its size. Contrary to miRNAs which are 21-23 nucleotides in length, siRNAs which are 20-25 nucleotides in length, and piRNAs which are 24-31 nucleotides in length, rasiRNAs are 24-29 nucleotides in length depending on the organism it came from. Unlike siRNA which are derived from both the sense and antisense strand, rasiRNA are derived from the antisense. Interestingly, while miRNA requires Dicer-1 for its production, and siRNA requires Dicer-2, rasiRNA does not require either; however, in some plants there are Dicer-like (Dcl) proteins that have been identified where Dcl1 produces 24 nucleotide miRNA and siRNA while Dcl2 produces 24 nucleotide rasiRNA. This research shows that not only is rasiRNA production distinct from miRNA and siRNA, but that rasiRNA may be found in plants while Piwi proteins are not
rasiRNA are Distinct fro other RNAi pathways Unlike miRNA and sirNa which function through the ago Argonaute protein subfamily, rasirna is aso distinction ts Piw size ontrary to miRNAs which are 21-23 nucleotides in lengt th siRNAs which are 20-25 nucleotides in length, and pirnas which are 24-31 nucleotides in length, rasiRNAs are 24-29 nucleotides in length depending on the organism it came from. Unlike siRNA which are derived from both the sense and antisense strand rasiRNa are derived from the antisense Interestingly, while miRNa requires Dicer-l for its production, and siRNa requires Dicer-2, rasirna does not require either; however, in some plants there are Dicer-like dcl) proteins that have been identified where Dell produces 24 nucleotide miRNA and sirNa while dcl2 produces 24 nucleotide rasiRNA. This research shows that not only is rasiRNa production distinct from miRNA and sirna, but that rasiRNA may be found in plants while piwi proteins are not
RasiRNA are Distinct from other RNAi pathways Unlike miRNA and siRNA which function through the Ago Argonaute protein subfamily, RasiRNA function through the Piwi Argonaute protein subfamily. RasiRNA is also distinct in its size. Contrary to miRNAs which are 21-23 nucleotides in length, siRNAs which are 20-25 nucleotides in length, and piRNAs which are 24-31 nucleotides in length, rasiRNAs are 24-29 nucleotides in length depending on the organism it came from. Unlike siRNA which are derived from both the sense and antisense strand, rasiRNA are derived from the antisense. Interestingly, while miRNA requires Dicer-1 for its production, and siRNA requires Dicer-2, rasiRNA does not require either; however, in some plants there are Dicer-like (Dcl) proteins that have been identified where Dcl1 produces 24 nucleotide miRNA and siRNA while Dcl2 produces 24 nucleotide rasiRNA. This research shows that not only is rasiRNA production distinct from miRNA and siRNA, but that rasiRNA may be found in plants while Piwi proteins are not
染色质 植物hc DDCI HEN1 hc-s i RNA 21nt SIRNA 魂3 异染色质的形成涉 D准 及hc- siRNA(异染色 质 siRNA)它们的前 DNA甲基化与染色质重建 体从异染色质区转 complex Lysine 录,随后产生 dSRNA. dsRNA由DCL3 2K9) ( RNase ll)加工成 hc- SiRNA,之后转移 异染色 到将形成异染色质 hc-siRNA结合 染色质重建复合物 转录↓ dsRNA.合成RDR2 K9:赖氨酸 的区域,指导DNA的 甲基化和组蛋白的 甲基化酶和RSC复合物 ↓eocu 修饰.hc- SiRNA也 hc-s i RNA 称 Small scan rnas组蛋白8聚体 romain remodelling complex (ScnRNAS) MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Dec. 2003, p. 657-685
植物hcsiRNA 异染色质的形成涉 及hc-siRNA(异染色 质siRNA).它们的前 体从异染色质区转 录, 随后产生dsRNA. dsRNA由DCL3 (RNase III)加工成 hc-siRNA, 之后转移 到将形成异染色质 的区域, 指导DNA的 甲基化和组蛋白的 修饰. hc-siRNA 也 称Small scan RNAs (scnRNAs)