HybridizeFragment&Extend片段杂交与延伸AdaptersequenceSingle DNA libraries are接头序列hybridizedtoprimerlawn变性后单链DNA文库与流动槽上的引物杂交Bound libraries are thenextendedbypolymerases随后在DNA合成酶的作用下，结合上的DNA文库进行延伸Surfaceofflowcellcoatedwithalawnofoligopairs流动槽表面随机植入了大量引物链3'extension3延伸
DenatureDouble-Stranded DNA双链DNA变性3333NewlysynthesizedDouble-strandedstrandmoleculeisdenaturedOriginal新合成链template双链DNA分子被变性原始模板链Original templatewashed瑞去Discardaway原始的模板链被洗去Newlysynthesized strandiscovalentlyattachedtoflow cell surface新合成的DNA链以共价键连接的方式结合在流动槽表面