Typical Biological Lab Functions ·Synthesis ·Analysis A A+B◆ B A+B Mixing Reaction Separation 6
Typ g ical Biological Lab Functions • Synthesis • Analysis A B C A B A + B A + B Mixing Reaction Separation 6
Microfluidics 。 Continuous-flow biochips:Permanently etched microchannels, micropumps and microvalves Digital microfluidic biochips:Manipulation of liquids as discrete droplets ELECTRODES GLASS (Duke University) SLCON Mixed Droplet Photodiode 2002 PC BOARD Electrical Pad GEL CHANNELS 86 PHOTODETECTORS WIRE BONDS HEATERS 88 LED ELUIDIC CHANNELS Mixing Sample AIR VENTS Electrowetting Electrode Reagent Control AIR LINES (University of Michigan) electronics 1998 (shown)are suitable for Printed circuit board eWI到 handheld or lab-on-a-chip- benchtop inexpensive and applications readily manufacturable 1111111期 7
Microfluidics • Continuous-flow biochips: Permanently etched microchannels, micropumps and microvalves • Digital microfluidic biochips: Manipulation of liquids as discrete droplets (Duke University) 2002 (University of Michigan) 1998 Control electronics (shown) are suitable for suitable for handheld or benchtop applications Printed circuit board lab-on-a-chip – inexpensive and readily manufacturable 7
What is Digital Microfluidics? Discretizing the bottom electrode into multiple electrodes,we can achieve lateral droplet movement Continuous Electrode Top Plate Discrete Electrodes Note:oil is typically used to fill between the Droplet Transport(Side View) top and bottom plates to prevent evaporation. 8
What is Digital Microfluidics? • Discretizing the bottom electrode into multiple electrodes, we can achieve lateral droplet movement achieve lateral droplet movement Note: oil is typically used to fill between the Droplet Transport (Side View) t d b tt l t t t ti 8 top and bottom plates to prevent evaporation
What is Digital Microfluidics? Transport 25 cm/s flow rates, order of magnitude higher than continuous-flow methods For videos,go to www.ee.duke.edu/research/microfluidics 9
What is Digital Microfluidics? Transport 25 cm/s flow rates, order of magnitude higher than higher than continuous-flow methods F id t d k d / h/ i fl idi 9 For videos, go to www.ee.duke.edu/research/microfluidics
What is Digital Microfluidics? Splitting/Merging 10
What is Digital Microfluidics? Splitting/Merging 10