Tissue culture culture and incubation Plant Physiology Experiments
Plant Physiology Experiments Tissue culture —— culture and incubation
tissue culture Explants o A technique which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium o By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels
tissue culture zA technique which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. zBy manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels. Explants
o Cultures of Organised structures MEristem cultures O Shoot tip, or shoot cultures O Node cultures of separte lateral buds ISolated root cultures O Embryo cultures o Cultures of Unorganised tissues O Callus(or tissue)cultures O Suspension (or cell)cultures O Protoplast cultures O Anther cultures Plant Tissue Culture Procedure- Background by e.f. george
z Cultures of Organised structures {Meristem cultures {Shoot tip, or shoot cultures {Node cultures of separte lateral buds {Isolated root cultures {Embryo cultures z Cultures of Unorganised tissues {Callus (or tissue) cultures {Suspension (or cell) cultures {Protoplast cultures {Anther cultures Plant Tissue Culture Procedure – Background. By E.F. George
How to Choice of Explants material correctly? ● Position o Season ●Aae Size Induction rate dedifferentiation and redifferentiation capacity
How to Choice of Explants Material correctly? zPosition zSeason zAge zSize Induction rate： ——dedifferentiation and redifferentiation capacity
The principal methods of micropropagation Meristem culture Alcr burning M tile ae cry shoot One o mom rode ct shoot Soral Ad.anne DIRECT shoots Direct Explants fern Indirect shoot STOCK PLANT someti Issua formalion INDIRECT punters MORPHOGENESIS Calus grow Cats Cavermous Indirect embryogenesis Smote embryos clue
The principal methods of micropropagation