Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and,if so, how closely. Recombination fraction Centimorgan,cM
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely. ❖ Recombination fraction ❖ Centimorgan, cM
Genetic Marker A genetic marker is a tag which allows to identify a place in a genome (locus),is a 'tag' near or within a gene of interest.Genetic markers are usually highly polymorphic
Genetic Marker A genetic marker is a tag which allows to identify a place in a genome (locus), is a ‘tag’ near or within a gene of interest. Genetic markers are usually highly polymorphic
Genetic Marker Blood group;Serum protein;HLA *DNA RFLP DNA STR DNA VNTR Single nucleotide polymorphism,SNP
Genetic Marker ❖ Blood group; Serum protein; HLA ❖ DNA RFLP ❖ DNA STR & DNA VNTR ❖ Single nucleotide polymorphism, SNP
Linkage Analysis Neurofibroma,NF RFLP EcoR I EcoR I EcoR I 3.0kb —1.7kb Probe
Linkage Analysis EcoRⅠ EcoRⅠ EcoRⅠ 3.0 kb 1.7 kb Probe Neurofibroma, NF1 RFLP Ⅰ Ⅱ Ⅲ 1 2 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8
Linkage Analysis Neurofibroma,NF RFLP 5 6 8 3.0kb -1.7kb 4.7kb
Linkage Analysis Neurofibroma, NF1 RFLP Ⅰ Ⅱ Ⅲ 1 2 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 3.0 kb 1.7 kb 4.7 kb