CHAPTER& The replication of nA The mechanism of DNA Polymerase (Pol) Copyright 2004 Pearson Education, Inc, publishing as Benjamin Cummings @博共字 Wuhan Universit
The mechanism of DNA Polymerase (Pol) CHAPTER 8 The replication of DNA
DNA Pol use a single active site to catalyze DNA synthesis A single site to catalyze the addition of any of the four dNTPs. Recognition of different dNTP by 2 monitoring the ability of incoming dNTP in forming A-T and G-c base pairs; incorrect base pair dramatically lowers the rate of catalysis( kinetic selectivity)
DNA Pol use a single active site to catalyze DNA synthesis A single site to catalyze the addition of any of the four dNTPs. Recognition of different dNTP by monitoring the ability of incoming dNTP in forming A-T and G-C base pairs; incorrect base pair dramatically lowers the rate of catalysis (kinetic selectivity). The mechanism of DNA Pol
a correct base pair b incorrect base pair template C-0 C no base p X dNTP dNTP OH OH OH OH primer O=P-0 Distinguishing different dNTPs: kinetic selectivity Copyright o 2004 Pearson Education, InC, publishing as Benje Fiqure 8-3
Figure 8-3 Distinguishing different dNTPs: kinetic selectivity
Distinguishing between rNTP and dNTP by steric exclusion of rNTPs from the active site 030339 template c-0 discriminance dNP O P-0 DNA polymerase Fic gure 8-4
Distinguishing between rNTP and dNTP by steric exclusion of rNTPs from the active site. The mechanism of DNA Pol Figure 8-4
DNA Pol resemble a hand that grips the primer-template junction Schematic of DNA pol bound to a MACA primer:template junction 30339 A similar view of the T7 DNA pol Tq bound to DNA Fiqure 8-5 Copyright o 2004 Pearson Education, Inc, publishing as Benjamin Cummings
DNA Pol resemble a hand that grips the primer-template junction The mechanism of DNA Pol Figure 8-5 Schematic of DNA pol bound to a primer:template junction A similar view of the T7 DNA pol bound to DNA