CHAPTER& The replication of nA Teaching Arrangement Watch animation-Understand replication a Go through some structural tutorial-Experience the BEAUTY of the DNA polymerase a Lecture-comprehensive understanding and highlight Key points
Teaching Arrangement ◼ Watch animation-Understand replication ◼ Go through some structural tutorial-Experience the BEAUTY of the DNA polymerase ◼ Lecture-comprehensive understanding and highlight Key points CHAPTER 8 The replication of DNA
CHAPTER& The replication of nA The chemistry of DNA Synthesis The Mechanism of DNA Polymerase > The Specialization of DNA Reaction Polymerases catalyst >The Replication Fork Process DNA Synthesis at the Replication Fork >Initiation of DNA Replication Initiation Binding and Unwinding Termination >Finishing Replication
➢ The Chemistry of DNA Synthesis ➢ The Mechanism of DNA Polymerase ➢ The Specialization of DNA Polymerases ➢ The Replication Fork ➢ DNA Synthesis at the Replication Fork ➢ Initiation of DNA Replication ➢ Binding and Unwinding ➢ Finishing Replication Process Initiation & Termination CHAPTER 8 The replication of DNA Reaction & Catalyst
CHAPTER& The replication of nA The first part describes the basic chemistry of DNA synthesis and the function of the dna polymerase @博共字 Wuhan Universit
The first part describes the basic chemistry of DNA synthesis and the function of the DNA polymerase CHAPTER 8 The replication of DNA
CHAPTER& The replication of nA The chemistry of DNA DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction DNA is synthesized by extending the 3 end of the primer Hydrolysis of pyrophosphate(PPi is the driving force for dNA synthesis @博共字 Wuhan Universit
The Chemistry of DNA CHAPTER 8 The replication of DNA ➢ DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction ➢ DNA is synthesized by extending the 3’ end of the primer ➢ Hydrolysis of pyrophosphate (PPi) is the driving force for DNA synthesis
base(A, G, C, or T) Ho—P—0-P-—P—0-CH2 OH b annealed primer growing end of DNA 50 OH 3 template strand ACGT CGACGTCGACGTCGACGT 3 HO 5 dsDNA SSDNA Figure 8-3 Substrates required for DNA synthesis
Figure 8-3 Substrates required for DNA synthesis