The recycled organic biomass (compost) was composed by thefollowing organic residues45% urban solid waste 40% plant residues (tobacco) 15%plant trimmingthe recycled organic biomass was produced through acomposting process made of a common oxidation period (activephase) of 30 days, followed a stabilization period (curing phase) of of120 days30 t ha-l of recycled organic biomass were added to soil for 4 yearssoil humic acids were then extracted from soil at the followingintervals4th year (HA 0 year) 5th year (HA 1 year) 6th (HA 2 year)IREEA-NanjingAgriculturalUniversity/April2008
The recycled organic biomass (compost) was composed by the following organic residues 45% urban solid waste 40% plant residues (tobacco) 15%plant trimming the recycled organic biomass was produced through a composting process made of a common oxidation period (active phase) of 30 days, followed a stabilization period (curing phase) of of 120 days 30 t ha-1 of recycled organic biomass were added to soil for 4 years soil humic acids were then extracted from soil at the following intervals 4th year (HA 0 year) 5th year (HA 1 year) 6th (HA 2 year) IREEA-Nanjing Agricultural University/ April 2008
200 g soilHumic acid extractionSoxhlet extractionwith ethyl ether1M NaOH + 0.1MNaP,0-+centrifugation 7000 rpmshaken overnight ( 1 liter)pelletsfiltration quartz filter 0.45 μsupernatantwashing withpH 1 with HClhumic acidsdeionized watertill pH7fulvic acidspurification with→ dialysisHF/HCl solutionIREEA-Nanjing AgriculturalUniversity/April2008
200 g soil Soxhlet extraction with ethyl ether 1M NaOH + 0.1M Na4P2O7 shaken overnight ( 1 liter) supernatant pellets centrifugation 7000 rpm washing with deionized water till pH7 filtration quartz filter 0.45 μ purification with HF/HCl solution dialysis pH 1 with HCl Humic acid extraction fulvic acids humic acids IREEA-Nanjing Agricultural University/ April 2008
Solid state 13C-NMR CPMASHA O year6 CHOHDCH,OHcelluloseligninH15ORRO福HHH-HOHOHproteinsHHHHCH,327327HOHHOHOHlipidsMeoOMe76RNHQR-(CH2)nC36lignin66-62-CH,HH89-84106Alkyl acids /MeOOMe58/oxidisizedchainsOR0173134153R-C-OH150500100200IREEA-NanjingAgriculturalUniversity/April2008
O CH2OH RO H H H OH OH H H O O CH2OH H H H OH OH H H OR O CH2OH RO H H H OH OH H H O O CH2OH H H H OH OH H H OR cellulose 56 OMe OR MeO O R H H lignin 200 150 100 50 0 Solid state 13C-NMR CPMAS HA 0 year 6 6 66-62 4 4 89-84 106 1 1 76 3 2 5 5 3 2 36 -(CH2)n- -CH3 lipids 56 NH2 O OH CH3 proteins 134 OR MeO OMe O R H H 153 lignin 173 R-C-OH O Alkyl acids / oxidisized chains IREEA-Nanjing Agricultural University/ April 2008
IREEA-NanjingAgricultural13C-NMR of soil humic acidsUniversity/April2008relative decreaserelativeincreaseyearA0HA 1 yearHA 2 year150502001000
13C-NMR of soil humic acids HA 0 year HA 2 year 200 150 100 50 0 HA 1 year relative increase relative decrease IREEA-Nanjing Agricultural University/ April 2008