NCBI protein-protein BLAST Nctn区 low(BLASTi] Rara optons lo vMed blow or IMba troa[ one), g]空 Confer E Low cospa Mask tow loon) tabb ouly Mw lowr a Meu[PAM7014Gn Con [Emstnce. 7Emmen:
Psi-BLAST Position-specific iterative BLAST Combines BLAST searching with PSSMs! 1- Start with regular BLAST search- look at the results
Psi-BLAST Position-specific iterative BLAST Combines BLAST searching with PSSMs! 1- Start with regular BLAST search – look at the results
Distribution of 42 Blast Hits on the Query sequence Mouse-over to show de fline and scores. Click to show alignments Color Key for Alignnent Scores 〈40 40-50 50-80
Psi-BLAST Position-specific iterative BLAST Combines BLAST searching with PSSMs! 1- Start with regular BLAST search- look at the results 2-Pick the ones you believe are really homologous
Psi-BLAST Position-specific iterative BLAST Combines BLAST searching with PSSMs! 1- Start with regular BLAST search – look at the results 2- Pick the ones you believe are really homologous
sequences w ith E-va loe HE ItER than threshold Sequences producing signi ficant alignments Value hw a siL14795405lreEIXP_ 947240,11 () polo-like kinase(Droso..623 四区叫4B269161 reEINP 00S02l,l(0030)po0lo-1 ike kinase( Drosop,,522 H B gi13930171gb1MAA5653411 (001030) pLX Homo sapiens) 521e-177 Hw B gi14034581sblAAA36559,11(L19559) protein kinase Homo sapiens I 519e-176 H E si1s75210alreEINP.35251,11 (NM011121) polo-like kinase homolog.,500e-171 4 2 stL1093470lpirl1A4754% protein kinase (EC 2.7. 1 37) PIk- mouse e-170 H 2 sil122303961sp10676731PLK 1 RAT Serine/threonine-protein kinase P 592e-170 区s13597351|AK2859,1Ar3021:(A39021pol0-1 like prote.,.4e-154 区叫1s304lMc01,(58205)px×1( Xenopus1 devisE e-142 区sl1463974曲hBA1B59,1(A043897)1p010-1 ke kinas( Hemicen,,.36236-99 is B gi14657831splP343311Yx24 CMEEL Hypothetical 41. 8 kDa protein C14..293 2e-78 y sil1755-4392lreEINP 498770,11 (NM 066369) Protein kinase (Caeno 2932e-78 y sil30636451gb 129.l (AF057165) putative serine/threonine p.. 293 2e-7B m区s1510519 reEINP410351cm059635)Y719, a Caenorhabda,,.2021e-75 n E s 17737679lre INP 524179.11(NM 079455) polo (Drosophila melano 2516e-67 R E giL142861671splP52304180I2_ DROME PROTEIN KINASE POLo >gil7293666 i 0 gil33667921gblAAC28624,11(AF033092) polo-like kinase iso for (R..15236-36 a M sil17541716re EINP 501196 11 (NM 068795) protein kinase (Caenorh,.1502e-35 i a si15730055IreEINP_ 006613 1| (NM 006622)serusinducible kinase [.. 11 Ie-32 M sil14230424lre fI_041712 11 (XM_041712) serun-inducible kinase.. 140 1e-32 M siLAz116sm, SNK MUSE Serine/threonine-protein kinase s 1393e-32 m区silk3 u7alresINP_1140091L(m f 031821) serun-inducible kinase 1395e-32 区s16023川clMa017,15721(Ar357042)po10-1 like kin4e.,.116-32 i a sil169073671sb1AA30175 1 AF357840 1( AF357840) polo-like kinase. 1324-31 n a gi18339101gblAAC52191, Al (021392) putative serine/threonine kina.. 137e-31 y gi138784401sp1Q60806ICNK_ NDUSE CYTOKINE-INDUCIBLE SERINE/THREON 1347e-31 N9 2 gi14759016lre INP 004064,11 (NM 004073) cytokine-inducible kinas e-30