Directed evolution of thermos table esterases Differential scanning calori metry of eight generati ons of evol ved enzymes a0000 800 8 Tm=695 △0000 vld type Tm=525 20000 555 84 65 7 Tem perature (c)
Catalytic activities of evolved thermophilic'esterases 08 Generation 8 Evolved enzyme is 07 more actve over af 0月 temperatres 篮兰 05 04 03 02 wiLdtype A 0.1 0 3 5日 7 Te mperature°
成功实例 Lipase genes (lipB52, lipB68) isolated from Pseudomonas fluorescens b52、B68 Genbank Accssion number AY623009- AY694785 含 Nucleotide 匚D±=p1ay「dfau1t how:20 v toF±1 Get Subsequen seudomonas f 2T-丁UN-2004 AY623009.1G工149036109 aeudomonadeoteeb paeudoimonagaproteobacteria: Pseudomonadales 个1。2113P。an。11D2xCm Len, Y.. Lou, Y. d Ma, Y TOURNAL Submitted ci teHx。b1°Ea ursyeraxtv ar s五H1Es1° eh⊥ma NCBI NUcleotid h Nucleotid. Go Clear D玉ep1ay ←道1t snow,=o-1Cs。na。(xx □ Featur。a 11合器,m 三五上一2口4 rot-abaet-r: Pender a计”量3“1,x:m:ra” udomonastluo an8, Z.. MO. Y. and we3. D. 1工
Lipase genes (lipB52, lipB68) isolated from Pseudomonas fluorescens B52、B68 Genbank Accssion number AY623009、AY694785