(二)荧光显微镜Fluorescence microscope 特点:光源为短波光: 有两个特殊的滤光片; 照明方式通常为落射式。 Dr Tian 2008
(二)荧光显微镜Fluorescence microscope • 特点:光源为短波光; • 有两个特殊的滤光片; • 照明方式通常为落射式
eyepiece 3 3 second barrier filter:cuts out unwanted fluorescent signals passing the specific green fluorecein emission between 520 and 560nm LIGHT SOURCE 2 beam-splitting mirror:reflects light below 510nm but transmits light above 510nm 1 first barrier filter:lets through only blue light with a wavelenght objective lens between 450 and 490nm object
用于观察能激发出荧光的结构。用途:免疫荧光 观察、基因定位、疾病诊断。 Fluorescence image,DNA in blue and Microtubules in green Dr Tian 2008
Fluorescence image, DNA in blue and Microtubules in green • 用于观察能激发出荧光的结构。用途:免疫荧光 观察、基因定位、疾病诊断
(三)激光共聚焦扫描显微境 Laser confocal scanning microscope,LCSM 用激光作光源,逐点、逐行、逐面快速扫描: ,能显示细胞样品的立体结构; ·分辨力是普通光学显微镜的3倍; ·用途类似荧光显微镜,但能扫描不同层次,形成 立体图像。 D7e2008
(三)激光共聚焦扫描显微境 Laser confocal scanning microscope, LCSM • 用激光作光源,逐点、逐行、逐面快速扫描; • 能显示细胞样品的立体结构; • 分辨力是普通光学显微镜的3倍; • 用途类似荧光显微镜,但能扫描不同层次,形成 立体图像
laser confocal scanning microscope,LCSM 1.Confocal scan head 2.Upright microscope stand 3.High-precision z-stage 4.Anti-vibration table 10 5.Control panel 6.Contral console 7.Computer table 8.Supply unit withlasers and electronics 9.Box with kryptonlaser 10.Monitor 11.PC workstation
laser confocal scanning microscope, LCSM