●Baseline(基线)Chromatographic curve under the experimental operation conditions, no components of materials flow out after the column(在实验操作条件下, 色谱柱后没有样品组分流出时的流出曲线称为基线,反 映检测器系统噪声(Noise) 随时间的变化情况。稳定的 基线应该是一条水平直线。) Nomenclature of Chromatographic separation ●Baseline drift(基线漂移)refer to 基线随时间定向 的缓慢变化 ●Peak height refer to Vertical distance from the vertex of chromatographic peak to baseline 色谱峰顶点与基线之 间的垂直距离,以(h)表示
●Baseline(基线)Chromatographic curve under the experimental operation conditions, no components of materials flow out after the column(在实验操作条件下, 色谱柱后没有样品组分流出时的流出曲线称为基线,反 映检测器系统噪声(Noise) 随时间的变化情况。稳定的 基线应该是一条水平直线。) Nomenclature of Chromatographic separation ●Baseline drift(基线漂移)refer to 基线随时间定向 的缓慢变化 ●Peak height refer to Vertical distance from the vertex of chromatographic peak to baseline 色谱峰顶点与基线之 间的垂直距离,以(h)表示
Nomenclature of Chromatographic separation ▲Chromatogram and chromatographic peak ▲ Baseline(a) ▲ Peak height(h) 信 号 进样 空气峰 色谱峰 h a 色谱流出曲线
Nomenclature of Chromatographic separation ▲Chromatogram and chromatographic peak ▲ Baseline(a) ▲ Peak height(h) 信 号 进样 空气峰 色谱峰 h a 色谱流出曲线
●Retention value 表示试样中各组分在色谱柱中滞留时间的数值。通常用 时间或用将组分带出色谱柱所需载气的体积来表示。 ■ Rely on component partition on two phases (or absorption) ■Thermodynamic controlled (Properties of stationary phase & operation conditions) ■ Qualitative Base Nomenclature of Chromatographic separation ● Dead Time tM 不被固定相吸附或溶解的物质(如空气,甲烷等)进 入色谱柱时,从进样到出现峰极大值(浓度)所需的时 间称为死时间,它正比于色谱柱的空隙体积。见图
●Retention value 表示试样中各组分在色谱柱中滞留时间的数值。通常用 时间或用将组分带出色谱柱所需载气的体积来表示。 ■ Rely on component partition on two phases (or absorption) ■Thermodynamic controlled (Properties of stationary phase & operation conditions) ■ Qualitative Base Nomenclature of Chromatographic separation ● Dead Time tM 不被固定相吸附或溶解的物质(如空气,甲烷等)进 入色谱柱时,从进样到出现峰极大值(浓度)所需的时 间称为死时间,它正比于色谱柱的空隙体积。见图
●Rotention time tR The time at which the maximum of the peak appears after sample injection 见图 Nomenclature of Chromatographic separation 因为这种物质不被固定相吸附或溶解,故其流动 速度将与流动相流动速度相近。测定流动相平均线速 ū时,可用柱长L与tM的比值计算,即 ū = L/tM
●Rotention time tR The time at which the maximum of the peak appears after sample injection 见图 Nomenclature of Chromatographic separation 因为这种物质不被固定相吸附或溶解,故其流动 速度将与流动相流动速度相近。测定流动相平均线速 ū时,可用柱长L与tM的比值计算,即 ū = L/tM
Nomenclature of Chromatographic separation ●Adjusted rotention time(tR ´) The difference between the rotention time and dead time 某组分的保留时间扣除死时间后,称为该组分的调整保留 时间,即 tR ´= tR − tM • More time the component spends due to dissolution in (or adsorption on) the stationary phase than inert components without interaction. 保留时间是色谱法定性的基本依据,但同一组分的保留 时间常受到流动相流速的影响,因此色谱工作者有时用 保留体积来表示保留值
Nomenclature of Chromatographic separation ●Adjusted rotention time(tR ´) The difference between the rotention time and dead time 某组分的保留时间扣除死时间后,称为该组分的调整保留 时间,即 tR ´= tR − tM • More time the component spends due to dissolution in (or adsorption on) the stationary phase than inert components without interaction. 保留时间是色谱法定性的基本依据,但同一组分的保留 时间常受到流动相流速的影响,因此色谱工作者有时用 保留体积来表示保留值