Chapter 2 Gas Chromatography(GC) §2-1 Summary ●Chromatographic separation technique(色谱分离): A separation technique based on partition of 2 or more substances in a mixture in two phases(色谱法、色层法、 层析法,chromatographer) ● Chromatographic analysis(色谱分析): Chromatographic separation applied on analytical chemistry Advantages: High separation efficiency, high detection efficiency, fast analytical velocity GC
Chapter 2 Gas Chromatography(GC) §2-1 Summary ●Chromatographic separation technique(色谱分离): A separation technique based on partition of 2 or more substances in a mixture in two phases(色谱法、色层法、 层析法,chromatographer) ● Chromatographic analysis(色谱分析): Chromatographic separation applied on analytical chemistry Advantages: High separation efficiency, high detection efficiency, fast analytical velocity GC
●Mobile phase & Stationary phase(流动相与固定相): Chromatographic separation involving partition of different species in a mixture in two phases, among which one phase (solid or liquid) does not move (stationary phase) , the other phase (gas or liquid) takes the sample mixture to pass through the stationary phase packed in the column( mobile phase) Separations are affected due to the differences of substances’ affinities for a mobile phase and a stationary phase(分离原理). GC
●Mobile phase & Stationary phase(流动相与固定相): Chromatographic separation involving partition of different species in a mixture in two phases, among which one phase (solid or liquid) does not move (stationary phase) , the other phase (gas or liquid) takes the sample mixture to pass through the stationary phase packed in the column( mobile phase) Separations are affected due to the differences of substances’ affinities for a mobile phase and a stationary phase(分离原理). GC
(a) Sample Mobile phase A+B Packed column A B Detector 2
Gas supply Flow controller Oven Column To waste Detector Flow meter (In one of these positions) Injection system Amplifier Integrator Recorder GC
Gas supply Flow controller Oven Column To waste Detector Flow meter (In one of these positions) Injection system Amplifier Integrator Recorder GC
皂膜流量计 转字流量计 调整仪,标 准仪 Setup of Gas Chromatograph GC
皂膜流量计 转字流量计 调整仪,标 准仪 Setup of Gas Chromatograph GC