有效放大倍率 Resolution of naked eye Effective magnification= Resolution of microscope Effective magnification (Men: resolution of eye 0.2mm ≈1333× max.resolution of LOM 0.00015mm M's >Merr make the image bigger,but do not provide any additional details.This is termed "empty magnification. 6
有效放大倍率 6
显微镜系统 Reflected Light ·物镜 Binocular Lamp House Head Aperture Diaphragm Field Collector ·光学系统 Eyepieces Diaphragm Lens Vertical ·照明系统 llluminator Filter Slider ·记录系统 Objective- Internal Mechanical Circuit Board Stage ● 载物台 Condenser 。控制系统 Base Field Diaphragm Filters Transmitted Light Lamp House 7
显微镜系统 • 物镜 • 光学系统 • 照明系统 • 记录系统 • 载物台 • 控制系统 7
显微镜的镜头物镜 Image formation in a reflected light microscope lllumination Similar to transmission source optical microscope. Different arrangement of components Condenser aperture and lens Half-silvered (dichromatic) mirror B Object 放大倍数 Final (specimen) First 数值孔径 Image Image Objective 分辨率 aperture and lens Depth of field 物镜 像差校正 物镜类型 8
显微镜的镜头/物镜 8 • 放大倍数 • 数值孔径 • 分辨率 • Depth of field • 像差校正 • 物镜类型 物镜
像差(aberration) 。 在轴像差 一色差、球差 。 离轴像差 一慧差、像散 ·畸变 一场曲 -桶形/枕形畸变 9
像差(aberration) • 在轴像差 – 色差、球差 • 离轴像差 – 慧差、像散 • 畸变 – 场曲 – 桶形/枕形畸变 9
60x Plan Apochromat Objective Nosepiece Mounting Manufacturer Thread Flat-Field、 Aberration JAPAN Correction Nippon Correction Plan Apo Lateral Numerical Magnification- 60×/1.40oi1、 Aperture DIC H Specialized /0.17WD0.21、 Immersion Optical Medium Properties Working Tube Length Distance Magnification Color Code Coverslip Thickness Spring-Loaded Retraction Stopper Figure 1 10
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