Effect of laminar shear stress on cell alignment(Dewey etal.1981) Photos removed due to copyright considerations See Dewey, C F, et al. The dynamic response of vascular endothelial cells to fluid shear stress J Biomech Eng. 1981 Aug; 103(3): 177-85 No flow aminar shear stress
Effect of laminar shear stress on cell alignment (Dewey et al. 1981) Photos removed due to copyright considerations. See Dewey, C.F., et al. "The dynamic response of vascular endothelial cells to fluid shear stress." J Biomech Eng. 1981 Aug;103(3):177-85. No flow Laminar shear stress�
applied external force mechano sensitive ior conformational chann structural proteins Signaling altered △ In protein synthesis or post expression translational modifications
applied external force mechano- conformational sensitive ion changes in channel structural proteins Signaling cascade altered D gene synthesis or post expressionn translational modifications in protein
Image removed due to copyright considerations Hanahan Weinberg, Cell [20001 We know quite a lot about the signaling cascade that follows the initial biochemical event, leading to morphological changes, variations in various biochemical signals changes in gene expression and protein synthesis, but relatively little about how the initial event is transduced from physical force to biochemical reaction
Image removed due to copyright considerations. Hanahan & Weinberg, Cell [2000] We know quite a lot about the signaling cascade that follows the initial biochemical event, leading to morphological changes, variations in various biochemical signals, changes in gene expression and protein synthesis, but relatively little about how the initial event is transduced from physical force to biochemical reaction
Mechanotransduction Hair cell stimulation stereocilium removed due to copyright considerations ension in the tip link activates a SEM of the stretch-activated Image removed due to stereocilia on the copyright considerations ion channel, leading to intracellular surtace of a single hair cell (hudspeth calcium ion fluctuations
Mechanotransduction: Hair cell stimulation stereocilium tip link tension in tip link increases Image removed due to copyright considerations. SEM of the Image removed due to stereocilia on the copyright considerations. surface of a single hair cell (Hudspeth) Tension in the tip link activates a stretch-activated ion channel, leading to intracellular calcium ion fluctuations
Structure of a mechano-Sensitive lon Channel (Mscl, large conductance) Chang et al. science 1998 Membrane stresses alter pore geometry and control the exchange of ions Images removed due to copyright considerations
Structure of a Mechano-Sensitive Ion Channel (MscL, large conductance) Chang, et al., Science, 1998 Membrane stresses alter pore geometry and control the exchange of ions Images removed due to copyright considerations