Chapter 2. Cell-Matrix Interactions. [that determine biomaterials function in vitro and in vivo] A. How cells pull onto and deform the matrix to which they attach themselves. B. Cell-matrix interactions control the spontaneous closure of wounds in organs. C. What happens when regeneration is induced?

UNIT CELL PROCESSES Concept of a \Control Volume\ around a Cell Soluble Regulator Cell Matrix

CELLS FOR TISSUE ENGINEERING/REGENERATIVE MEDICINE Autologous -Differentiated parenchymal cells of same or other tissue type -Stem cells (adult) Allogeneic

TISSUE ENGINEERING What is tissue engineering Production of tissue in vitro by growing cells in porous, absorbable scaffolds (matrices). Why is tissue engineering necessary? Most tissues cannot regenerate when injured or diseased

SACHUSE Massachusetts Institute of Technology Harvard Medical School Brigham and Women's/Massachusetts General Hosp

C. Synthesis of biologically active scaffolds (regeneration templates) 1. History of biologically active scaffolds (regeneration templates). 2. Physical chemistry of collagen: Melting of collagen quaternary structure (thromboresistance). Melting of collagen tertiary structure (gelatinization). 3. Synthesis of ECM analogs: lonic complexation

The Extracellular Matrices Part. 2. Elastin fibers. 3. Proteoglycans(PG) and glycosaminoglycans (GAG). 4. Cell-adhesion molecules (CAM)

RESPONSE TO IMPLANTS: WOUND HEALING Surgical Implantation Inc. time Vascular Response Acute Clotting Inflammation Phagocytosis Granulation Neovascularization New Collagen Synthesis Tissue

biologically active model of ECM acts as an insoluble regulator of cell function image removed due to copyright considerations

RESPONSE TO IMPLANTS WOUND HEALING flury Vascular Response Inflammation Tissue of labile Tissue of and stable cells Permanent cells Framework framework Scar Intact Destroyed