《生物化学》课程PPT教学课件：引言——蛋白质概述

第一节 概述 第二节 酵母菌 第三节 霉菌

第一节 病毒的形态结构与化学组成 第三节 病毒的主要类群与繁殖方式

Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer

Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?

Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??

实验步骤 (1)确定罐上发酵培养基的配制、灭菌及 接种方式对发酵的影响。 (2)查资料确定你选定的发酵产品在发酵 过程中的主要影响因素,如还原糖的量、 pH的波动,并将其转换为可以调整的物质 如需要添加葡萄糖调节还原糖的量,通过 添加氨水或尿素来调节pH及氨的供给