F E oop o 30-nm chromatin D fiber A。oB C Chromosome scaffold A FIGURE 9-36 Experimental demonstration of chromatin loops in interphase chromosomes. In situ hvbridization of
high-level looped domain expressio folded of genes loc fiber histone modifying enzymes chromatin remodeling complexes RNA polymerase proteins forming chromosome scaffold Figure 4-44 A model for the structure of an interphase chromosome. A section of an in chromosome is shown folded into a series of looped domains, each containing 20,000-100,000 nu r-LI.L-lt--I ANIA dened inen 20 nm fiher Individual loons ran recondense perha
ure 12.13 Chromatin loops: a higherlevel of chromatin ucture. (a) Electron micrograph of a mitotic chromosome t had been treated with a solution of dextran sulfate to re- ve histones. The histone-depleted chromosome displays ps of DNA that are attached at their bases to a residual pro- a scaffold. (6)The looped chromatin domains are also appar in this micrograph of a lampbrush chromosome isolated m an amphibian oocyte. (A: FROM JAMES R PAULSON AND <LAEMMLI CELL 12: 823, 1977; BY PERMISSION OF CELI PRESS, OUKTESY OF JOsEPH GG
igure 4-54 An electron micrograp f a mitotic chromosome. This
chromosome 1400nm 300nr Beads or o chromatin 八八m A AGURE 9-35 Model for the packing of chromatin and the