Living Cells in Brightfield and Phase Contrast (a) Figure 2 (b) 11
11
P wave S wave Incident beam,λ D wave 111 Ad<<A 20 △ (b) Vectors 2π6 4π△d S Wave 9 二 P Wave D Wave 如何将相位差转变为亮度差？ 12
12 B A d S P S P D Incident beam, d<<
(a) Frits Zernike(1888-1966) First described in 1934 Nobel Prize in Physics 1953 S Wave P Wave D Wave Phase。 CI Positive Vectors POS +入 P Brightfield Images Negative NEG %4入 O D Figure 6 13
13 Frits Zernike (1888-1966) First described in 1934 Nobel Prize in Physics 1953