Isolation & Culture: ColonySizeShapeColorSurface featuresSmooth - Rough TransparencyTranslucent colonytype Hemolysis
Isolation & Culture: Colony Size Size Shape Shape Color Color Surface features Surface features Smooth Smooth - Rough Rough Transparency Transparency Hemolysis Hemolysis
Biochemical ReactionsH2S TestCitrate utilizationSugar Fermentation石河子大学病原生物学与免疫学教研室
Biochemical Reactions Sugar Fermentation H Sugar Fermentation H22S Test Citrate utilization S Test Citrate utilization 石河子大学病原生物学与免疫学教研室
Serological AssaysDetection antibody in the patient's serumAcurrent infection should beIgM posiveA4-foldorgreaterriseonantibodytiterbetweentheacuteserumsample and the convalescent serum sampleMajor drawbacksAsinglelgGantibodytiterisdfficult tointerpretbecauseitisunclearwhether it represents a current or a previous infectionthe convalescent sample is usuallvtaken 10-14 davs after the acutesample.By this time,the patienthas often recovered andthe diagnosisbecomesa retrospective oneSome exceptionsIncertaindiseasesasingletiterofsufficientmagnitudecanbeuedpresumptive evidence of a current infection石河子大学病原生物学与免疫学教研室
Serological Assays Detection Detection antibody antibody in the patient in the patient’’s serum A A current infection current infection should be should be IgM positive positive A 4 A 4-fold or greater rise on antibody titer between the acute serum fold or greater rise on antibody titer between the acute serum sample and the convalescent serum sample sample and the convalescent serum sample Major Major drawbacks drawbacks A single A single IgG antibody titer is difficult to interpret because it is unclear antibody titer is difficult to interpret because it is unclear whether it represents a current or a previous infection whether it represents a current or a previous infection the convalescent sample is usually taken 10 the convalescent sample is usually taken 10-14 days after the acute 14 days after the acute sample. By this time, the patient has often recovered and the di sample. By this time, the patient has often recovered and the diagnosis agnosis becomes a retrospective one becomes a retrospective one Some Some exceptions exceptions In certain diseases, a single titer of sufficient magnitude can In certain diseases, a single titer of sufficient magnitude can be used as be used as presumptive evidence of a current infection presumptive evidence of a current infection 石河子大学病原生物学与免疫学教研室
AnimalexperimentsAnimal experiment AnimalsMouseGuinea Pig Rabbit Dog MonkeyInoculation routesIntradermalSubcutaneousIntraperitonealIntravenousIntracranial / intracerebralIntraspinalIntranasalLavage石河子大学病原生物学与免疫学教研室
Animal experiment Animals Animals Mouse Mouse Guinea Pig Guinea Pig Rabbit Dog Monkey Rabbit Dog Monkey Inoculation routes Inoculation routes Intradermal Intradermal Subcutaneous Subcutaneous Intraperitoneal Intraperitoneal Intravenous Intravenous Intracranial / Intracranial / intracerebral intracerebral Intraspinal Intraspinal Intranasal Intranasal Lavage Lavage 石河子大学病原生物学与免疫学教研室
Virulence Test Median lethal dose, LD50ID50Median infective dose.Elek Plate DiphtherotoxinCorynebacterium diphtheriaeEnterotoxinenterotoxigenic E.coli石河子大学病原生物学与免疫学教研室
Virulence Test Median lethal dose, Median lethal dose, LD50 LD50 Median infective dose, Median infective dose, ID50 ID50 Elek Elek Plate Plate Diphtherotoxin Diphtherotoxin Corynebacterium diphtheriae diphtheriae Enterotoxin Enterotoxin enterotoxigenic enterotoxigenic E. coli E. coli 石河子大学病原生物学与免疫学教研室