Transporter Spokes Cytoplasmic filaments cytoplasmic particles gure 12. 6 A model a vertebrate nuclear pore complex
e no um Figure 12. 5 Scanning electron micrographs of the nuclear pore complex from isolated nuclear envelopes of an amphib
NIC96 NUP159 CYT· 匙 Symmetric Cytoplasmic igure 12.7 The molecular architecture of the yeast nuclear pore complex. To identify the locations of each of the 30 or so ucleoporins that comprise the yeast nuclear pore complex, re- searchers prepared a subcellular fraction that was highly en riched in NPCs, extracted the nucleoporins, purified the indi NUCL vidual proteins by column chromatography and gel electrophoresis, and prepared antibodies against each of the pu
NIC96 NUP159 CYT Symmetric Cytoplasmic Figure 12.7 The molecular architecture of the yeast nuclear ore complex. To identify the locations of each of the 30 or so searchers prepared a subcellular fraction that was high/,re- cleoporins that comprise the yeast nuclear pore compl d in NPCs, extracted the nucleoporins, purified the indi vidual proteins by column chromatography and gel NUCL electrophoresis, and prepared antibodies against each of the pu 9宫8
4. Nuclear lamina Underlying the inner nuclear membrane is the nuclear lamina. a electronic dense fibrous mesh work The filaments of the nuclear lamina are composed of polypeptides, called lamins. Most mammalian cells contain four different lamins designated A. Bla B2 and C All the lamins are 60-80 kd fibrous proteins that related to the intermediate filament proteins of the cytoskeleton The lamina associate with each other to form filaments(10 nm in diameter), first to form dimers in a coiled coil structure by winding the a-helical regions of two lamin chains around each other These dimers then associated with each other by head-to- tail association of dimers and side-by-side association of polymers to form the filaments that make up the nuclear lamina
4.Nuclear lamina Underlying the inner nuclear membrane is the nuclear lamina, a electronic dense fibrous meshwork. The filaments of the nuclear lamina are composed of polypeptides, called lamins. Most mammalian cells contain four different lamins designated A, B1 , B2 and C. All the lamins are 60-80 kd fibrous proteins that related to the intermediate filament proteins of the cytoskeleton. The lamina associate with each other to form filaments(10 nm in diameter), first to form dimers in a coiled coil structure by winding the -helical regions of two lamin chains around each other.These dimers then associated with each other by head-to- tail association of dimers and side-by-side association of polymers to form the filaments that make up the nuclear lamina