AIMS o Determine how pathways of 5-ht metabolism are affected by the gut microbiota o Identify specific microbial communities and factors involved in conferring serotonergic effects o Evaluate how microbial modulation of peripheral 5-HT impacts host physiology
AIMS Determine how pathways of 5-HT metabolism are affected by the gut microbiota. Identify specific microbial communities and factors involved in conferring serotonergic effects. Evaluate how microbial modulation of peripheral 5-HT impacts host physiology
RESULTS elevated by Sp and hSp
RESULTS elevated by Sp and hSp
GF MICE EXHIBIT SIGNIFICANTLY DECREASED LEVELS OF seRUM colonic 5-ht A n s ★★★ 1.25 3.0 1.00 生2 0.75 1.5 10 0.25 05 0.00 0. SPF GE PO P21 P42 Po P21 P42 P42 SPF GF PO P21 P42 PO P21 P42 P42 GF+CONV SPF+ABX VEH GF+CONV. SPF+ABX VEH
GF MICE EXHIBIT SIGNIFICANTLY DECREASED LEVELS OF SERUM & COLONIC 5-HT
1000 o 5-hT are localized to colonic 8000 chromogranin A-posotive( CgA+) 600 enterochromaffin cells(ECs)(p +oo 铬粒蛋白A阳性的肠嗜铬细胞) SPF GF P21 P42 P21 P42 GFGF +Sp +Sp F+CONv.SPF十ABX +PCPA CgA+ 5-HT SPF GF
5-HT are localized to colonic chromogranin A-posotive (CgA+) enterochromaffin cells (ECs)（嗜 铬粒蛋白A阳性的肠嗜铬细胞）
o Which are the specific steps of 5-HT metabolism that are affected by the microbiota? o TPHl: the rate-limiting enzyme for 5-ht biosynthesis in ECS O SLC6A4: 5-hT transporter
Which are the specific steps of 5-HT metabolism that are affected by the microbiota? TPH1: the rate-limiting enzyme for 5-HT biosynthesis in ECs SLC6A4: 5-HT transporter