The confocal microscope Scan excitation spot point- Detector by-point to build up image Pinhole Tube lens Excitation light Emission light Objective lens Sample
The confocal microscope Sample Objective lens Excitation light Tube lens Emission light Pinhole Detector Scan excitation spot point- by-point to build up image
How big should your pinhole be? Want pinhole to pass entire Airy disk X Airy disk diameter ≈1.22元/NA Width of point spread function at pinhole: Airy disk diameter x magnification of lens
How big should your pinhole be? Want pinhole to pass entire Airy disk Airy disk diameter ≈ 1.22 /NA Y X Width of point spread function at pinhole: Airy disk diameter × magnification of lens