实验一 培养基的配制与灭菌 一 教学要求 ⚫ 通过配制牛肉膏蛋白胨等培养基，掌握配制培 养基的一般方法和步骤。 ⚫ 了解采用的灭菌方法和原理，掌握高压蒸汽灭 菌技术。 二 实验原理 1、Media Types ⚫ Solid Media,semi-solid Media, liquid Media . ⚫ Enriched Media, Selective Media, Differential Media， combination selective and differential media
实验一 培养基的配制与灭菌 一 教学要求 ⚫ 通过配制牛肉膏蛋白胨等培养基，掌握配制培 养基的一般方法和步骤。 ⚫ 了解采用的灭菌方法和原理，掌握高压蒸汽灭 菌技术。 二 实验原理 1、Media Types ⚫ Solid Media,semi-solid Media, liquid Media . ⚫ Enriched Media, Selective Media, Differential Media， combination selective and differential media
2 Sterilization – destruction of all forms of microbial life, including endospores. Physical Methods —Moist heat Autoclaving • Action - protein denaturation. • Very effective method of sterilization; at about 15 psi（Ib/in2 ） of pressure (121ºC [ 250ºF]), all vegetative cells and their endospores are killed in about 15 min
2 Sterilization – destruction of all forms of microbial life, including endospores. Physical Methods —Moist heat Autoclaving • Action - protein denaturation. • Very effective method of sterilization; at about 15 psi（Ib/in2 ） of pressure (121ºC [ 250ºF]), all vegetative cells and their endospores are killed in about 15 min
0
Autoclave Steam and pressure high temp~121C ↑team exhaust Chamber pressure Valve (C) aive (E) gauge- A Steam exhaust Thermometer Trap Pressure Steam ontera(Aj】 Fig.21.5 regulator
—Dry heat Direct flaming • example: Inoculation loop Hot-air sterilization • Baking in oven • Very effective method of sterilization, but requires temperature of 170ºC [ 340 ºF]for about 2 hr
—Dry heat Direct flaming • example: Inoculation loop Hot-air sterilization • Baking in oven • Very effective method of sterilization, but requires temperature of 170ºC [ 340 ºF]for about 2 hr