Exons from different RNA molecules can be fused by g Trans-splicing 4 - Trans-splicing: the process in 9 Which two exons carried on oo different RNA molecules can be spliced together
Exons from different RNA molecules can be fused by Trans-splicing ◼ Trans-splicing: the process in which two exons carried on different RNA molecules can be spliced together. The chemistry of RNA splicing
Trans-splicing RNA I RNA‖ exon 1 exon 2 5 GUAAG3′ AAG OH Fiqure 13-5 AAG Not a lariat 5′ 3′ Copyright C 2004 Pearson Education, Inc, publishing as Benjamin Cummings
Trans-splicing Figure 13-5 Not a lariat
CHAPTER 13 RNA Splicing Topic 2 THE SPLICESOME MACHINERY
Topic 2 THE SPLICESOME MACHINERY CHAPTER 13 RNA Splicing
3 RNA splicing is carried out by a o large complex called spliceosome Q The above described splicing of Q introns from pre-mRNA are mediated by the spliceosome n The spliceosome comprises about 150 proteins and 5 o SnRNAS. ■ Many functions of the 3 spliceosome are carried out by its RNA components
RNA splicing is carried out by a large complex called spliceosome ◼ The above described splicing of introns from pre-mRNA are mediated by the spliceosome. ◼ The spliceosome comprises about 150 proteins and 5 snRNAs. ◼ Many functions of the spliceosome are carried out by its RNA components. The spliceosome machinery
a The five RNAs (U1, U2,U4, U5, and U6,100-300 nt) are called small nuclear RNAs(snRNAS). a The complexes of snRNA and proteins are called small nuclear ribonuclear proteins(snRNPI pronouncesSnurps 95 a The spliceosome is the largest snRNP, and the exact makeup differs at different stages of the splicing reaction
◼ The five RNAs (U1, U2, U4, U5, and U6, 100-300 nt) are called small nuclear RNAs (snRNAs). ◼ The complexes of snRNA and proteins are called small nuclear ribonuclear proteins (snRNP, pronounces “snurps”). ◼ The spliceosome is the largest snRNP, and the exact makeup differs at different stages of the splicing reaction