5′、RNA B DNA RNAP R-loop 5 stop codon Ribosome Rho NusG Gowrishankar. J and harinarayanan. R. 2004. Why is transcription coupled to translation in bacteria? Mol Microbiol. 54:598-603 Do you have some ideas on eukaryotes?
Gowrishankar, J. and Harinarayanan, R. 2004. Why is transcription coupled to translation in bacteria? Mol. Microbiol. 54: 598-603. Do you have some ideas on eukaryotes?
Niu DK(2007). Protecting exons from deleterious R-loops: a potential advantage of having introns Biology Direct 2: 11 5 <√RNA DNA Polymerase RNA RNA RNA RNA RNA DNA Polymeras Polymerase- Polymerase c 5 5 3 RNA DNA Polymerase
Niu DK (2007). Protecting exons from deleterious R-loops: a potential advantage of having introns. Biology Direct 2: 11
RNA polymerase 5 RNA 3 ZTTTTHTTT 5 Transcript cleavage 5′DNA proteins can prevent stalling of a Backtracking backtracked polymerase OIIIIIITTIITIIT RNA cleavage to prevent stalling
Transcript cleavage proteins can prevent stalling of a backtracked polymerase
Stringent response in poor growth conditions Reduce to 5% of the normal level (A)ppGpp (B)pppGpp 0-P-0-P-0—cH2 o-P-0-P-0-P-0—cH2 o H o=P-0 o=P-0 o=P-0 0=P-0
Stringent response in poor growth conditions • Reduce to 5% of the normal level
Something New Transcription WT cells Base J Base J levels termination by AVAWAWAWAWAWAWAWAAMAW base j JBP2 KO cells Readthrou Base Jk Hazelbaker Dane Z, BuratowskiS 2012 Current Biology Transcription base j block Figure 1. DNA base J prevents transcriptional readthrough in Leishmania. the Way. Curr Bio/ 22: R960 R2
Something New • Transcription termination by base J. • Hazelbaker Dane Z, Buratowski S. 2012. Transcription: Base J Blocks the Way. Curr Biol 22: R960- R2