In 1981, embryonic stem cells(Es cells) were first derived from mouse embryos by martin Evans In 1998 James Thomson first developed a technique to isolate and grow in cell culture human embryonic stem cells Martin Evans, Cambridge Univ James a thomson Wisconsin UnIV USA
11 Martin Evans， Cambridge Univ. In 1981, embryonic stem cells (ES cells) were first derived from mouse embryos by Martin Evans. In 1998, James Thomson first developed a technique to isolate and grow in cell culture human embryonic stem cells. James A Thomson,Wisconsin Univ.USA
内细胞团 囊胚 畸胎瘤 胎儿 胚胎干细胞胚胎生殖细胞 胚胎癌细胞 多能干细胞 The routes from which the embryonic stem cells may be obtained
12 The routes from which the embryonic stem cells may be obtained
体外培养时,细胞排列紧密,呈集落状生长 细胞克隆和周目存在明显界限。 细胞克隆形态多样,多数呈岛状或巢状 The cultured human em bryonic stem cells 13
13 The cultured human embryonic stem cells 体外培养时，细胞排列紧密，呈集落状生长。 细胞克隆和周围存在明显界限。 细胞克隆形态多样，多数呈岛状或巢状
胚胎千细胞 胚状体 无饲养层培养 悬浮培养 The differentiation in Vitro of em bryonic stem cells 14
14 The differentiation in Vitro of embryonic stem cells
通过流式细胞仪 选 进行分离 选择性培基筛飞性标志筛选 HSCS 神经细 肌肉细胞 研制组织工程 组织或者器官 组织或器官移植 The induction of embryonic stem cells and stem cell engineering
15 The induction of embryonic stem cells and stem cell engineering