一)接合现象的发现 Bio'PheCys'Thr Leu Thi Bio Phe Cys Thr'Leu'Thi' Porous cotton plug Mixture Strain A Strain B 接合子 Fine filter Bio'Phe'Cys'Thr'Leu'Thi Prototrophic colonies 6
6 一）接合现象的发现 接合子
Bacterium Mobile Plasmid Pilus 二)接合过程 Chromosome 2. Donor Recipient 3. Relaxosome Transferosome 4. New Donor New Donor
7 二）接合过程
有关菌株 F- 雌性（female) male(donor F+ ucleoi Hfr 雄性（(male) male (dond 8
8 有关菌株 F- F+ Hfr F’ × × 雌性（female） 雄性（male）
1.F+XF杂交 F+菌株的F因子向F细胞转移 Dono Retained strand DNA polymerase 结果？ Primer Unwinding protein Plasmid-encoded membrane proteins walls Specific outer memb ane protein of recipient Transferred strand DNA polymerase Primer 9 Recipient
9 1. F+×F-杂交 F +菌株的F因子向F -细胞转移 结果？
2.Hfr XF杂交 「因子的先导区结合着染色 体DNA向受体细胞转移 结果？ Recombination F recipient pro pro man pro A E D C B cys 94
10 2. Hfr ×F-杂交 F因子的先导区结合着染色 体DNA向受体细胞转移 结果？