0 群体总DNA 用通用或专一性引物 扩增1 S rRNA基因 2 3 全部16S 不同16S rRNA基因 rRNA基因 回收条带 基因克隆和测序
群体总DNA 用通用或专一性引物 扩增16S rRNA基因 全部16S rRNA基因 不同16S rRNA基因 1 2 3 4 1 2 3 4 回收条带 基因克隆和测序
(a) DNA fragment Vector Recombinant vector (b) Recombinant vector E.coli (c) + + Antibiotic Antibiotic o0 E.coli without vectors dies E.coli with vectors proliferates 测序 (d)Isolation of recombinant DNA clones
测序
ddGTP ddATP ddCTP ddTTP
(a) Bacteria (b) Archaea Proteobacteria(122) Methanomicrobia③ -TM6 Thermoplasmata (3) OS-K Halobacteria (3) Termite group Sodimont Archaoa.】 Nitrospira pMC2 Euryarchaeota -Bacteroidetes(4) SAGMEGI Chlorobi (1) ☑pMC1 Marine group A WS3 了Termc ( Gemmimonas Firmicutes(52) Methanococcales(4) Thermoprotei (7) YNPFFA Synergistes Delerrbacteres 9 NKB19 C1() Chiamydiae (11) FCG1 -OP3 etes(2) Spirochaetes (5) FCG2 OP10 01 -TM7 Deinococcus-Thermus(3) Thormodosulfcbactora Thermotogae(2) OP11 GB.c 生物多样性和系统发育的多样性
生物多样性和系统发育的多样性
0.05 G2-6(AY28064512/58 Methanosaeta concilii (X16932) Methanosaeta thermophilum (AB071701) ΓG2-9(AY280647)1/581 Methanosarcina mazei(AE013440) 甲烷菌 -Methanosarcina barkeri(AJ012094) G3:14(AY280649)1/58 Archaea Methanocorpusculum parvum (AY057068) Methanobrevibacter curvatus (U62553) G3-20(4Y280653)2/58] Methanobacterium formicicum (AF028689) Methanobacterium subterraneum (X99045) G413(AY280655)13/58] Methanobacterium bryantii (AF028688) Methanobacterium oryzae(AF028690) Methanobacterium thermophilum (X99048) Methanococcus vannielii (M36507) Methanothermococcus thermolithotrophicus(M59128) Sulfolobus metallicus (X90479) 北京王致和食品集团有限公司废水反应器菌群16SRNA基因多样性 古菌
Archaea G2-6 (AY280645) [2/58] Methanosaeta concilii (X16932) 100 Methanosaeta thermophilum (AB071701) 100 G2-9 (AY280647) [1/58] Methanosarcina mazei (AE013440) 99 Methanosarcina barkeri (AJ012094) 100 95 G3-14 (AY280649) [1/58] Methanocorpusculum parvum (AY057068) 100 100 Methanobrevibacter curvatus (U62553) G3-20 (AY280653) [2/58] Methanobacterium formicicum (AF028689) Methanobacterium subterraneum (X99045) 100 84 G4-13 (AY280655) [13/58] Methanobacterium bryantii (AF028688) Methanobacterium oryzae (AF028690) 90 40 55 Methanobacterium thermophilum (X99048) 39 Methanococcus vannielii (M36507) 100 Methanothermococcus thermolithotrophicus (M59128) 100 63 Sulfolobus metallicus (X90479) 0.05 甲烷菌 北京王致和食品集团有限公司废水反应器菌群16S rRNA 基因多样性——古菌