Visualizing intracellular Ca2+ concentration by the fluorescent indicator fura-2 in a Purkinje cell 100um
Visualizing intracellular Ca2+ change by the fluorescent indicator fura-2 15 s after sperm added 0080 3 min 32s ...息 ca2+】1
ndirect immunofluorescence labeled Tchnique To study the location of a specific protein within the cell by using of fluorescent antibody (antigen-antibody couple). GFP can be used to study dynamic processes as they occur in a living cell. Dynamics of GFP tagging: follow the movement of spliceosome in the nucleus in a living cell
❖Indirect immunofluorescence lebeled Tchnique. To study the location of a specific protein within the cell by using of fluorescent antibody (antigen-antibody couple). GFP can be used to study dynamic processes as they occur in a living cell
Indirect immunocytochemistry primary antibody: secondary antibodies: rabbit antibody marker- coupled antibodies directed against directed against rabbit antigen A antibodies 4 marker immobilized antigen A Markers: fluorescence dyes, horseradish peroxidase, alkaline phosphatase, colloidal gold
Confocal Scanning Light Microscopy In the late 1950s M. minsky of mIT 共焦小孔 双色镜 物镜 样品 焦点 (A) (B) B C 免疫荧光技术(A)和激光共焦显微镜技术(B的比较 激光扫描共焦显微镜的原理图A激光束(光源)经双色镜反射后,通 过物镜汇聚到样品某一焦点;B.从焦点发射的荧光(样品一般须经免疫 荧光标记)经透镜汇聚成像,被检测器检出:C通过样品其它部位的激 光即激光发出的荧光不会聚焦成像,因而检测器不能检出
Confocal Scanning Light Microscopy 激光扫描共焦显微镜的原理图 A.激光束（光源）经双色镜反射后，通 过物镜汇聚到样品某一焦点；B.从焦点发射的荧光(样品一般须经免疫 荧光标记)经透镜汇聚成像，被检测器检出；C. 通过样品其它部位的激 光即激光发出的荧光不会聚焦成像，因而检测器不能检出。 In the late 1950s M.Minsky of MIT 免疫荧光技术（A）和激光共焦显微镜技术(B)的比较