干细胞分类 根据分化潜能不同 COMPARISON OF THE DIFFERENT SOURCES OF STEM CELLS 全能干细胞 Embryonic Stem Cells Adult Stem Cells 一多能干细胞 In Vitro Fertilization Nuclear Transter Adult Tissues 单能干细胞 Attributes cmposcoieln can produce all cell types demonstrated success in some treatments solate.mantain.and solate,maintain.and grow stem cels may be genetically In the laboratory matched to patlent 根据来源不同 Limitations lmited number of cel not yet achleved with human cels rsk of creating teratomas not found n all tissues 一胚胎干细胞 (tumois)from implanting lsk of creating teratomas undlfferentlated stem cels dlmcult to ldentity,isolate a090yamdgownt 一成体干细胞 destruction ot human destruction of human Ethical biastocysts biastocysts Concerns http://dels-old.nas.edu/bls/stemcells/types-of-stem-cells.shtml
干细胞分类 • 根据分化潜能不同 – 全能干细胞 – 多能干细胞 – 单能干细胞 • 根据来源不同 – 胚胎干细胞 – 成体干细胞 http://dels-old.nas.edu/bls/stemcells/types-of-stem-cells.shtml
单能干细胞 epithelial cell migration from "birth"at the bottom LUMEN OF GUT of the crypt to loss at the top of the villus villus (no cell division) (transit time is 3-5 days) cross section of villus epithelial cells crypt loose cross section brush orde of crypt cell goblet cells nondividing 小肠上皮微 differentiated cells 绒毛基部细 direction of cell movement 胞（黄色、 11 hours) 红色)。 slowly dividing es (cycle time 2 24 hours) A nondividing differentiated 1004m
单能干细胞 小肠上皮微 绒毛基部细 胞（黄色、 红色）
单能干细胞 dead flattened cells packed with keratin CELLS ARE SHED EPIDERMIS (epithelium)】 CELLS ARE DERMIS BORN (connective tissue) —30μm basal lamina dividing basal cell 皮肤的基底层细胞
单能干细胞 皮肤的基底层细胞
(三)胚胎干细胞 Blastocyst 受精卵 Inner cell mass 囊胚 stem cells 内细胞团 体外培养建系 A B
（三）胚胎干细胞
Producing stem cells from embryos and somatic cell nuclear transfer Normal Reproduction/IVF Derivation of Stem Cell Lines Somatic Cell Nuclear Transfer (SCNT) intent:Normal pregnancy Intent:Normal tissues for repa O Eam Early cell division Earlycell division s-7 days in cult时 /IV Fuilteem haby DtD4w和y d4 tributedto时e Centre
Producing stem cells from embryos and somatic cell nuclear transfer