In the lab, as little as a few picomoles (10-12 mol) of AfP can be measured using firefly luciferin and luciferase(荧光素酶每) using spectroscopIc methods. Inorganic polyphosphate could have served as an energy source in prebiotic and early cellular evolution
• In the lab, as little as a few picomoles (10-12 mol) of ATP can be measured using firefly luciferin and luciferase (荧光素酶), using spectroscopic methods. • Inorganic polyphosphate could have served as an energy source in prebiotic and early cellular evolution
0-P-O-P-0-P-0-RibAdenine 0-P-0-P-0-P-o-RibHAdenine H-0:-O ATP4- M H hydrolysis, with 0-P-o-P-O-RibHAdenine relief of charge repulsion MgADP O Mg O-P-OH +HO-P-0-P-O-RibHAde enine ADP resonance stabilization Ionizaton 3 O 60-P-08H H*+0-P-0-P-0-Rib ADenine O O ADP3- ATP+H2O一ADP-+P2-+H △G"°=-30.5kJ/mol